1. MicroRNA-519d targets MKi67 and suppresses cell growth in the hepatocellular carcinoma cell line QGY-7703
- Author
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Hou, Yan-Yan, Cao, Wei-Wei, Li, Lan, Li, Sheng-Ping, Liu, Tao, Wan, Hai-Ying, Liu, Min, Li, Xin, and Tang, Hua
- Subjects
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LIVER cancer , *NON-coding RNA , *CANCER cell growth , *CANCER cell proliferation , *BIOINFORMATICS , *MESSENGER RNA , *GROWTH factors , *PREVENTION - Abstract
Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that regulate the translation of target mRNA transcripts. In this study, we demonstrated that miR-519d was downregulated in human HCC and could suppress growth of the human HCC cell line QGY-7703. Bioinformatics analysis indicated that MKi67 was a putative target of miR-519d. In an EGFP reporter system, we confirmed that MKi67 was a direct target gene of miR-519d. Furthermore, knockdown of MKi67 inhibited QGY-7703 cell growth. These findings indicate that miR-519d targets the MKi67 transcript and suppresses HCC cell growth, suggesting that miR-519d has a tumor suppressive role in human HCC pathogenesis. [Copyright &y& Elsevier]
- Published
- 2011
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