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2 results on '"FACIM"'

1. Antimicrobial, antitrypanosomal and antibiofilm activity of Equisetum hyemale.

Author

Dos Santos Alves CF, Bonez PC, de Souza ME, da Cruz RC, Boligon AA, Piana M, Brum TF, Rossi GG, Jesus RD, Grando TH, Monteiro SG, Anraku de Campos MM, Giongo JL, and Vianna Santos RC

Subjects
Anti-Infective Agents isolation & purification, Chromatography, Liquid, Microbial Sensitivity Tests, Phytochemicals isolation & purification, Phytochemicals pharmacology, Plant Extracts isolation & purification, Anti-Infective Agents pharmacology, Bacteria drug effects, Biofilms drug effects, Candida drug effects, Equisetum chemistry, Plant Extracts pharmacology, Trypanosoma drug effects
Abstract

This study evaluates, for the first time, the antibiofilm, antimicrobial and antiparasitic potential of crude extract and fractions of stems of Equisetum hyemale against several infectious agents (bacteria, fungi, Mycobacterium and Trypanosomes) by broth microdilution technique and investigates the phenolic composition of the plant by high performance liquid chromatography. The crude extract and fractions showed antimicrobial activity, as they were capable of inhibiting the growth of bacteria in minimal inhibitory concentrations (MICs) ranging from 52.4 mg/mL to 3.27 mg/mL. For Candida species, the MICs ranged from 52.4 mg/mL to 6.5 mg/mL, and for Mycobacterium species from 2.5 mg/mL to 0.625 mg/mL. The dichloromethane fraction was able to reduce 83% of Pseudomonas aeruginosa biofilm formation and 51% of Candida albicans biofilms. The n-butanol fraction presents an important protozoal effect, reducing 100% of Trypanosoma evansi trypomastigotes after 9 h of exposure. The HPLC analysis revealed that the major substances are rosmarinic acid in dichloromethane fraction (7.38 ± 0.08 mg/g FS) and chlorogenic acid in ethyl acetate fraction (8.4 ± 0.26 mg/g FS). The crude extract and fractions of E. hyemale can be both useful and effective agents as a sustainable alternative for the treatment and prevention of several infectious agents., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

Published
2016
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2. Antimicrobial, antitrypanosomal and antibiofilm activity of Equisetum hyemale

Author

Silvia Gonzalez Monteiro, Thirssa H. Grando, Roberta da Silva Jesus, Marli Matiko Anraku de Campos, Pauline Cordenonsi Bonez, Roberto Christ Vianna Santos, Grazielle Guidolin Rossi, Janice Luering Giongo, Márcia Ebling de Souza, Mariana Piana, Ritiel Corrêa da Cruz, Camilla Filippi dos Santos Alves, Thiele Facim Brum, and Aline Augusti Boligon

Subjects
0301 basic medicine, Trypanosoma, Antiparasitic, medicine.drug_class, Equisetum, Phytochemicals, Ethyl acetate, Microbial Sensitivity Tests, Biology, 01 natural sciences, Microbiology, High-performance liquid chromatography, 03 medical and health sciences, chemistry.chemical_compound, Chlorogenic acid, Anti-Infective Agents, medicine, Food science, Candida albicans, Candida, Bacteria, Plant Extracts, Rosmarinic acid, Broth microdilution, Antimicrobial, biology.organism_classification, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, 030104 developmental biology, Infectious Diseases, chemistry, Biofilms, Chromatography, Liquid
Abstract

This study evaluates, for the first time, the antibiofilm, antimicrobial and antiparasitic potential of crude extract and fractions of stems of Equisetum hyemale against several infectious agents (bacteria, fungi, Mycobacterium and Trypanosomes) by broth microdilution technique and investigates the phenolic composition of the plant by high performance liquid chromatography. The crude extract and fractions showed antimicrobial activity, as they were capable of inhibiting the growth of bacteria in minimal inhibitory concentrations (MICs) ranging from 52.4 mg/mL to 3.27 mg/mL. For Candida species, the MICs ranged from 52.4 mg/mL to 6.5 mg/mL, and for Mycobacterium species from 2.5 mg/mL to 0.625 mg/mL. The dichloromethane fraction was able to reduce 83% of Pseudomonas aeruginosa biofilm formation and 51% of Candida albicans biofilms. The n-butanol fraction presents an important protozoal effect, reducing 100% of Trypanosoma evansi trypomastigotes after 9 h of exposure. The HPLC analysis revealed that the major substances are rosmarinic acid in dichloromethane fraction (7.38 ± 0.08 mg/g FS) and chlorogenic acid in ethyl acetate fraction (8.4 ± 0.26 mg/g FS). The crude extract and fractions of E. hyemale can be both useful and effective agents as a sustainable alternative for the treatment and prevention of several infectious agents.

Published
2016

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