1. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype.
- Author
-
de Sousa LVNF, Santos VL, de Souza Monteiro A, Dias-Souza MV, Marques SG, de Faria ES, Assunção EAO, Dos Santos SG, Zonis JM, de Alvarenga DG, de Holanda RA, de Sousa JG, Dos Santos KV, and Stoianoff MAR
- Subjects
- Antifungal Agents pharmacology, Biomarkers blood, Candida drug effects, Candida pathogenicity, Candidiasis, Oral blood, Candidiasis, Oral immunology, Candidiasis, Oral microbiology, Case-Control Studies, Cytokines blood, Gastrointestinal Neoplasms complications, Gastrointestinal Neoplasms microbiology, Humans, Laryngeal Neoplasms complications, Laryngeal Neoplasms microbiology, Microbial Sensitivity Tests, Mouth Neoplasms complications, Mouth Neoplasms microbiology, Opportunistic Infections blood, Opportunistic Infections immunology, Opportunistic Infections microbiology, Phenotype, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Candida isolation & purification, Candidiasis, Oral diagnosis, Gastrointestinal Neoplasms immunology, Laryngeal Neoplasms immunology, Mouth Mucosa microbiology, Mouth Neoplasms immunology, Opportunistic Infections diagnosis
- Abstract
Background: Because of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients., Methods: The yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay., Results: C. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17., Conclusions: The results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype.
- Published
- 2016
- Full Text
- View/download PDF