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Highlights • Girona province shows characteristics of a stable, endemic focus of CanL. • Estimated L. infantum infection seroprevalence for the province was 19.5%. • 93.2% of seropositive dogs were considered asymptomatic for CanL. • 27.3% of dog owners regularly used CanL prophylactic measures. • Dogs' age and altitude were identified as risk factors for L. infantum infection. • Results will improve risk assessment maps for CanL in the Mediterranean region. Abstract The Mediterranean basin is an endemic region for canine leishmaniosis (CanL), where it represents a major veterinary problem and raises human health concerns. However, the distribution of the disease is heterogeneous and not all countries and locations have been equally studied and characterized. This work describes the situation of CanL in Girona province (Catalonia, Spain), for which no data has been previously reported, and presents a relevant study to exemplify other areas with similar characteristics across the region. Four cross-sectional seroprevalence surveys were performed from 2012 to 2016 throughout the province, including 36 sampling stations in 26 localities and a total of 593 dogs. For each animal, individual and location variables were also collected. Additionally, each dog owner answered a questionnaire about their knowledge of CanL and preventive methods used. Blood samples were analysed by an in-house ELISA and a mixed logistic regression model was used to assess the relationship between pre-determined variables and dog seropositivity. A Spearman's correlation was used to assess the association between dog owners' perceived risk of CanL and Leishmania infantum seropositivity in dogs at a given location. The overall true seroprevalence estimated for Girona province was 19.5% (95%CI: 15.5–23.5), of which only 6.8% (10/146) were considered symptomatic. Age of the dog [OR = 1.21 (95%CI: 1.11-1.31); p < 0.001] and altitude [OR = 0.02 (95%CI: 0.001-0.19); p = 0.001] were identified as risk factors for the infection. The results obtained in this study are expected to aid in the implementation of directed control programmes in CanL endemic areas throughout Europe, as well as to provide suitable data for the design of better risk assessment maps of the disease. [ABSTRACT FROM AUTHOR]

Highlights: [•] Veterinary questionnaire on canine leishmaniosis in the Balearic Islands. [•] Prospective serological study on canine leishmaniosis in Minorca (Balearic Islands). [•] Entomological study of sand flies in Minorca: changes over time (1988 and 2009). [•] The risk factors associated to Phlebotomus perniciosus density. [•] Emergence of leishmaniosis in Minorca. [ABSTRACT FROM AUTHOR]

• No differences were detected in the number or severity of L. infantum active infections between vaccine and control groups. • No severe adverse reactions were observed in the dog population vaccinated with CaniLeish®. • Vaccination induced a significant rise in IFN-γ observable 1-month post-vaccination, which dropped after nine months. • Results fail to support the reported CaniLeish® vaccine efficacy in the prevention of active L. infantum infection. Dog vaccination is considered an effective way of reducing Leishmania infantum infection incidence in the canine population, as well as its transmission to humans. However, the use of partially effective vaccines can have the detrimental effect of "masking" vaccinated asymptomatic carriers, capable of harbouring the parasite and transmitting it to naïve individuals. After eight years on the European market, few studies have been released on CaniLeish® vaccine safety and efficacy. The present study, a one-year randomized CaniLeish® vaccine field trial, was performed in a canine leishmaniosis endemic area and included animals selected from a native dog population (n = 168). No severe adverse reactions were observed in vaccinated dogs (n = 85). Cases of active L. infantum infection were detected by serological, molecular and clinical follow-up of dogs. One-year post-vaccination, no differences in number or severity of L. infantum active infections were observed between study groups (n = 4 in each group). Vaccine-induced cellular immunity, assessed through interferon-γ quantification, showed significantly higher levels of this cytokine one-month post-vaccination in the vaccine group (p < 0.001), but no differences were observed after nine months between trial groups (p = 0.078). These results fail to support the reported CaniLeish® efficacy in the prevention of active L. infantum infection in dogs from endemic areas and naturally exposed to the parasite. Image, graphical abstract. [ABSTRACT FROM AUTHOR]