1. Mapping the N-linked glycosites of rice (Oryza sativa L.) germinating embryos.
- Author
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Ying, Jiezheng, Zhao, Juan, Hou, Yuxuan, Wang, Yifeng, Qiu, Jiehua, Li, Zhiyong, Tong, Xiaohong, Shi, Zhaomei, Zhu, Jun, and Zhang, Jian
- Subjects
RICE ,GERMINATION ,PLANT embryology ,GLYCOSYLATION ,POST-translational modification - Abstract
Germination is a key event in the angiosperm life cycle. N-glycosylation of proteins is one of the most common post-translational modifications, and has been recognized to be an important regulator of the proteome of the germinating embryo. Here, we report the first N-linked glycosites mapping of rice embryos during germination by using a hydrophilic interaction chromatography (HILIC) glycopeptides enrichment strategy associated with high accuracy mass spectrometry identification. A total of 242 glycosites from 191 unique proteins was discovered. Inspection of the motifs and sequence structures involved suggested that all the glycosites were concentrated within [NxS/T] motif, while 82.3% of them were in a coil structure. N-glycosylation preferentially occurred on proteins with glycoside hydrolase activities, which were significantly enriched in the starch and sucrose metabolism pathway, suggesting that N-glycosylation is involved in embryo germination by regulating carbohydrate metabolism. Notably, protein-protein interaction analysis revealed a network with several Brassinosteroids signaling proteins, including XIAO and other BR-responsive proteins, implying that glycosylation-mediated Brassinosteroids signaling may be a key mechanism regulating rice embryo germination. In summary, this study expanded our knowledge of protein glycosylation in rice, and provided novel insight into the PTM regulation in rice seed germination. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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