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1. A complete set of overlapping cosmid clones of M-ABA virus derived from nasopharyngeal carcinoma and its similarity to other Epstein-Barr virus isolates

Author

Axel Polack, Lutz Gissmann, Barbara Hohn, Gabriele Hartl, Takaki Kazutaka, U. Zimber, G Laux, Georg W. Bornkamm, and U K Freese

Subjects
Herpesvirus 4, Human, Carcinoma, fungi, Cloning vector, Nasopharyngeal neoplasm, food and beverages, Nasopharyngeal Neoplasms, General Medicine, Molecular cloning, Biology, Cell Transformation, Viral, Virology, Molecular biology, Virus, Restriction enzyme, Restriction map, Plasmid, DNA, Viral, Genetics, Cosmid, Cloning, Molecular, Plasmids
Abstract

DNA of the transforming, nondefective Epstein-Barr virus (EBV) strain M-ABA, which is derived from nasopharyngeal carcinoma cells, was cloned as large overlapping pieces into the cosmid pHC79 . The termini were cloned from closed circular virus DNA molecules out of M-ABA cell DNA in phage lambda L47 . The large overlapping clones were used to prepare a library of subclones with inserts of 1-15 kb. A detailed restriction enzyme map of M-ABA virus DNA reveals the close similarity to isolates from other sources. The high number of tandem repeats in EBV DNA stresses the importance of using cloning vectors that can be propagated in recA- Escherichia coli hosts.

Published
1984
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