1. Metal-derivatized major histocompatibility complex: zeroing in on contact hypersensitivity
- Author
-
Jacelyn, Loh and John, Fraser
- Subjects
inorganic chemicals ,Antigen Presentation ,Genes, MHC Class II ,Models, Immunological ,Receptors, Antigen, T-Cell ,Dermatitis, Contact ,Article ,Major Histocompatibility Complex ,nickel ,Metals ,hapten ,cardiovascular system ,Animals ,Humans ,hypersensitivity ,T cell receptor ,tissues ,Protein Binding - Abstract
The major histocompatibility complex (MHC) restriction element for a human Ni2+ reactive T cell, ANi-2.3, was identified as DR52c. A series of experiments established that the functional ligand for this T cell was a preformed complex of Ni2+ bound to the combination of DR52c and a specific peptide that was generated in human and mouse B cells, but not in fibroblasts nor other antigen processing–deficient cells. In addition, ANi-2.3 recognition of this complex was dependent on His81 of the MHC β chain, suggesting a role for this amino acid in Ni2+ binding to MHC. We propose a general model for Ni2+ recognition in which βHis81 and two amino acids from the NH2-terminal part of the MHC bound peptide coordinate Ni2+ which then interacts with some portion of the Vα CDR1 or CDR2 region.
- Published
- 2003