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Shigella spp. is the causative agent of gastric infections commonly acquired by the consumption of contaminated food. Chicken meat is an important transmission source of shigellosis due to its high demand and consumption. The purpose of this article is the detection of Shigella spp in chicken meat that is sold in Ambato from different markets. 135 samples were taken, 45 from places with an operating licence, 45 from informal food markets and 45 from slaughterhouses that supply the city of Ambato. Phenotypic identification was performed by culturing bacteria on selective medium, Gram staining and TSI. Shigella spp. candodates were sent for molecular identification by amplification of the 16S region of ribosomal DNA, where the presence of Shigella sonnei (10), Shigella flexneri (5) and Shigella dysenteriae (2) was demonstrated, whereas that Shigella boydii was not in any of the samples. In summary, three species of Shigella were identified, predominantly S. sonnei with greater inclination in slaughterhouses and informal outlets, which can mean a serious problem for Public Health. [ABSTRACT FROM AUTHOR]

Chicken meat is one of the most consumed proteins worldwide, however, it is one of the main reservoirs of Escherichia coli which is the main cause of multiple infections after consumption due to different factors such as inefficient handling at the time of slaughter, poor hygiene and inadequate refrigeration. The diseases it causes not only affect the health of consumers, but also the economy of traders. Therefore, this study aimed to identify by sequencing the 16S rRNA gene different strains that were isolated in chicken meat, for this 135 samples were taken and collected from formal and informal points of sale and from 9 slaughtering centers that supply Ambato. The samples were evaluated by means of microbiological and biochemical tests and then DNA was extracted from the 40 suspect samples, which were analyzed by PCR. The results were obtained through sequencing where a total of 33 different strains were identified, strains AF1 and ATCC 11775 were predominant in the chicken meat samples in the canton of Ambato. [ABSTRACT FROM AUTHOR]

Proteus mirabilis is a bacterium capable of causing pathologies of interest in public health. It occurs commonly in chicken meat. P. mirabilis does not stand out for its pathogenicity levels, but it does stand out for its difficult therapeutic approach once the infection has developed, due to its antibiotic resistance. In this study, 135 samples of chicken meat were collected in several retail centers in Ambato (45 formal sales, 45 informal sales and 45 slaughterhouses). Samples were seeded in selective culture media (McConkey Agar and XLD Agar), Gram staining was used, as well as biochemical tests (Oxidase Test and TSI) and molecular identification were performed by amplifying the 16S DNA ribosomal region, consequently there were 48 samples of P. mirabilis with 17 different strains, NCTC 11938 was the most common. P. mirabilis was mainly identified in formal retail centers, followed by slaughterhouses and informal retail centers. Meet contamination is related to food processing protocols breach. The great prevalence of this bacterium portrays a public concern issue, due to hazards related to meat contamination with it. [ABSTRACT FROM AUTHOR]