1. Mechanism of carotenoid coloration in the brightly colored plumages of broadbills (Eurylaimidae).
- Author
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Prum RO, LaFountain AM, Berg CJ, Tauber MJ, and Frank HA
- Subjects
- Animals, Chromatography, High Pressure Liquid, Color, Female, Magnetic Resonance Spectroscopy, Male, Spectrum Analysis, Raman, Tandem Mass Spectrometry, Carotenoids metabolism, Feathers metabolism, Passeriformes
- Abstract
The plumage carotenoids of six species from five genera of broadbills (Eurylaimidae) have been examined. These plumages are crimson, violet, purple-maroon, or yellow. Two genera also have brilliant green plumages that are produced by a combination of structural coloration and unknown carotenoids. Six different carotenoids from nine different plumage patches were identified, including two previously unknown molecules, using high-performance liquid chromatography, mass spectrometry, and MS/MS fragment analysis. The yellow pigment in Eurylaimus javanicus and Eurylaimus ochromalus is identified as the novel carotenoid, 7,8-dihydro-3'-dehydro-lutein. The yellow and green plumages of Psarisomus dalhousiae contain the unmodified dietary carotenoids lutein and zeaxanthin. The brilliant green feathers of Calyptomena viridis contain a mixture of lutein and two other xanthophylls that have previously been found only in woodpeckers (Picinae). The crimson and violet colors of Cymbirhynchus, Sarcophanops, and Eurylaimus are produced by a novel pigment, which is identified as 2,3-didehydro-papilioerythrinone. The molecular structure of this carotenoid was confirmed using (1)H nuclear magnetic resonance, correlated two-dimensional spectroscopy, and two-dimensional nuclear Overhauser effect spectroscopy. Resonance Raman (rR) spectroscopy carried out at room and low temperatures was used to probe the configuration and conformation of 2,3-didehydro-papilioerythrinone in situ within crimson C. macrorhynchos and purple-red E. javanicus feathers. The rR spectra reveal that the pigment is in an all-trans configuration and appears to be relatively planar in the feathers. The likely metabolic pathways for the production of broadbill carotenoids from dietary precursors are discussed.
- Published
- 2014
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