1. Neurite outgrowth-stimulating peptide derived from bovine kappa-casein.
- Author
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Sakaguchi M, Ishikawa C, Nishimura T, Sugihara E, and Matsumura E
- Subjects
- Amino Acid Sequence, Animals, Cattle, Cell Line, Tumor, Mice, Nerve Growth Factors chemistry, Neuroblastoma pathology, Caseins pharmacology, Neurites drug effects, Peptide Fragments pharmacology
- Abstract
We found a novel peptide that stimulates neurite outgrowth in Neuro-2a mouse neuroblastoma cells, from the pepsin-pancreatin digest of bovine kappa-casein. The amino acid sequence of this peptide is Phe-Leu-Pro-Tyr-Pro-Tyr (FLPYPY), corresponding to peptidic sequence 76-81 of bovine kappa-casein. The neurite outgrowth-stimulating activity of FLPYPY was seen over 10(-9) M. On the other hand, FLPYP and FLPYPYY, which corresponded to sequences 76-80 and 76-82 of bovine kappa-casein respectively, were ineffective. more...
- Published
- 2004
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