12 results on '"Xu, Yanan"'
Search Results
2. Proteomic analysis of coarse and fine skin tissues of Liaoning cashmere goat
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Bai, Zhixian, Xu, Yanan, Gu, Ming, Cai, Weidong, Zhang, Yu, Qin, Yuting, Chen, Rui, Sun, Yinggang, Wu, Yanzhi, and Wang, Zeying
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- 2022
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3. Association between the cashmere production performance, milk production performance, and body size traits and polymorphism of COL6A5 and LOC102181374 genes in Liaoning cashmere goats.
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Zhang, Yu, Qin, Yuting, Gu, Ming, Xu, Yanan, Dou, Xingtang, Han, Di, Lin, Guangyu, Wang, Lingling, Wang, Zhanhong, Wang, Jiaming, Sun, Yinggang, Wu, Yanzhi, Chen, Rui, Qiao, Yanjun, Zhang, Qiu, Li, Qian, Wang, Xiaowei, Xu, Zhiguo, Cong, Yuyan, and Chen, Jing
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MILK yield ,BODY size ,GENES ,CASHMERE ,ALCOHOL dehydrogenase ,SINGLE nucleotide polymorphisms - Abstract
The purpose of this study was to analyze the relationship between COL6A5 (collagen type VI alpha 5 chain) and LOC102181374 (alcohol dehydrogenase 1) genes and the production performance of Liaoning cashmere goats by single nucleotide polymorphism (SNP). We have searched for SNP loci of COL6A5 and LOC102181374 genes through sequence alignment and PCR experiments, and have used SPSS and SHEsis software to analyze production data. We obtained five SNP loci in total, including three SNP loci (G50985A, G51140T, G51175A) in COL6A5 gene and two SNP loci (A10067G, T10108C) in LOC102181374 gene. The genotypes G50985A (AG), G51140T (GT), G51175A (AA), A10067G (AA), and T10108C (CC) of these loci have certain advantages in improving the production performance of Liaoning cashmere goats. The haplotype combinations that can improve production performance in COL6A5 gene were H1H5:AGGGAG, H4H4:GGGGAA, and H4H4:GGGGAA. H3H3:GGCC and H2H4:AGTT were the dominant combinations in LOC102181374 gene. At G51175A and A10067G loci, we found that H1H2:AAAG and H1H3:AGAA have dominant effects. These results may provide some support for the molecular breeding of production traits in Liaoning cashmere goats. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Association analysis for SNPs of LIPE and ITGB4 genes with cashmere production performance, body measurement traits and milk production traits in Liaoning cashmere goats.
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Qiao, Yanjun, Gu, Ming, Zhang, Yu, Bai, Zhixian, Qin, Yuting, Xu, Yanan, Dou, Xingtang, Han, Di, Lin, Guangyu, Wang, Lingling, Wang, Zhanhong, Wang, Jiaming, Sun, Yinggang, Wu, Yanzhi, Chen, Rui, Zhang, Qiu, Li, Qian, Wang, Xiaowei, Xu, Zhiguo, and Cong, Yuyan
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MILK yield ,CASHMERE ,SINGLE nucleotide polymorphisms ,BODY composition ,GOAT breeds ,GENES - Abstract
Liaoning cashmere goat (LCG) is one of the excellent cashmere goat breeds in China. Because of its larger size, better cashmere, and better cashmere production performance, people pay special attention to it. This article mainly studied the relationship between SNP loci of LIPE gene and ITGB4 gene and milk production, cashmere production and body measurement traits of LCGs. We further identified potential SNP loci by PCR-Seq polymorphism detection and gene sequence comparison of LIPE and ITGB4 genes. Further, we use SPSS and SHEsis software to analyze their relationship to production performance. The consequence indicated that CC genotype of LIPE gene T16409C locus was dominant genotype in milk production and cashmere production, while CT genotype of LIPE gene T16409C locus was dominant in body size. The CT genotype of C168T locus of ITGB4 gene is the dominant genotype of body type and cashmere production, while the dominant genotype of milk production is TT genotype. Through joint analysis, in haploid combinations, H1H2:CCCT is the dominant haplotype combination in cashmere fineness. H3H4:TTCT is a dominant haplotype combination of milk production traits and body measurement traits. These dominant genotypes can provide a reliable basis for the study of production performance of LCG. [ABSTRACT FROM AUTHOR]
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- 2023
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5. Association analysis for SNPs of BAAT and COL1A1 genes with cashmere production performance and other production traits in Liaoning cashmere goats.
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Cai, Weidong, Xu, Yanan, Bai, Zhixian, Lin, Guangyu, Wang, Lingling, Dou, Xingtang, Han, Di, Wang, Zhanhong, Wang, Jiaming, Zhang, Xinjiang, Zhang, Yu, Qin, Yuting, Gu, Ming, Sun, Yinggang, Wu, Yanzhi, Chen, Rui, and Wang, Zeying
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SINGLE nucleotide polymorphisms , *CASHMERE , *GENES , *MILK yield , *HAPLOTYPES , *BODY composition - Abstract
This study aimed to investigate the relationship between the polymorphism of bile acid-CoA: amino acid N-acyltransferase (BAAT) and collagen type I alpha 1 chain (COL1A1) genes and the production performance of Liaoning Cashmere goat (LCG). The potential single nucleotide polymorphisms (SNPs) of LCG were detected by sequence comparison of BAAT and COL1A1 genes and PCR-Seq polymorphism, and the effect of SNPs on production performance was analyzed by SPSS software. The results showed that three SNPs loci were detected in BAAT gene: G7900A, T7967C, C7998T, and one SNP locus T6716C was detected in COL1AL gene. At G7900A locus, the dominant genotype for cashmere performance was GG, and the dominant genotype for body measurement traits and milk production traits was AG. At T7967C locus, the dominant genotype for cashmere performance was TT, and the dominant genotype for body measurement traits and milk production traits was CC. At C7998T locus, TT was the dominant genotype for cashmere performance, body measurement traits, and milk production traits. At the T6716C locus, TT was the dominant genotype for cashmere performance, body measurement traits, and milk production traits. H1H1: AACC is the dominant haplotype combination. Therefore, this study will provide a reliable reference for future research on cashmere production performance, body measurement traits, and milk production traits of LCG. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Association analysis between reproduction genes INHA, PGR, RARG with lamb and other traits of Liaoning cashmere goats.
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Chen, Rui, Liu, Jichang, Zhang, Yurou, Cai, Weidong, Zhang, Xinjiang, Xu, Yanan, Dou, Xingtang, Wang, Zhanhong, Han, Di, Wang, Jiaming, Lin, Guangyu, Wang, Lingling, Sun, Yinggang, Bai, Zhixian, Gu, Ming, and Wang, Zeying
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GOATS ,SINGLE nucleotide polymorphisms ,CASHMERE ,GOAT breeds ,LAMBS ,REPRODUCTION ,SHEEP breeding ,SHEEP farming - Abstract
Reproductive traits have a high economic value in goat breeding, and increasing the number of lambs produced by ewes is of great importance to improve the production efficiency of goat farming. Lambing traits in goats are low heritability traits, but their genetic basis is ultimately determined by genes. This study aimed to investigate the relationship between INHA, RARG, and PGR gene polymorphisms and production performance, such as lambing, cashmere production, milk production, and body size in Liaoning cashmere goats. A total of six single nucleotide polymorphisms (SNPs) loci were identified in these three genes, G144A and T504C on the INHA gene, A56G, G144A, G490C on the RARG gene, and G109519T on the PGR gene. For lambing and cashmere production traits, the AA genotype of G144A on the INHA gene, TT on the T504C genotype, GG genotype of G144A on the INHA gene, A56G, G144A, and T504C on RARG and G109519T on PGR gene are dominant genotypes. AATT is a dominant haplotype combination. Allele G can be used as a molecular marker for lambing, cashmere, and milk production traits in Liaoning cashmere goats. Marker-assisted selection can be used for early selection to achieve improvement of genetic traits in Liaoning cashmere goats. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Association analysis for SNPs of MSTN and IGFBP-3 genes with body size and other production traits in Liaoning Cashmere Goats.
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Sun, Yinggang, Cai, Weidong, Zhang, Xinjiang, Hui, Taiyu, Xu, Yanan, Han, Di, Dou, Xingtang, Wang, Zhanhong, Wang, Jiaming, Lin, Guangyu, Wang, Lingling, Hao, Jianjun, Fu, Shuqing, Wu, Yanzhi, Chen, Rui, Qin, Yuting, Zhang, Yu, Gu, Ming, Bai, Zhixian, and Wang, Zeying
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SINGLE nucleotide polymorphisms ,CASHMERE ,GENES ,HAPLOTYPES ,GOATS - Abstract
Liaoning cashmere goat (LCG) have tall bones, high cashmere production and outstanding meat production performance. In recent years, good breeding progress has not been made in terms of body size, meat yield, milk yield and other properties in terms of production. The study focused on the correlation between the SNPs of MSTN and IGFBP-3 genes with the body size performance, cashmere production and milk performance. The MSTN and IGFBP-3 gene sequence alignment and PCR-Seq polymorphism were used to detect the potential SNPs, and the correlation with production performance was analyzed by SPSS and SHEsis software. The results showed that the TT genotype at the T1662G locus of the MSTN gene is dominant and has significant advantages in body measurements such as sacrum height, chest width, and waist height. The C allele at the C4021T locus of IGFBP-3 gene shows an advantage in the body measurement performance. Among the haplotype combinations, H2H2:TGTC is preponderant combination for body size performance, H2H2:TGTC and H1H2:TGCC are preponderant combinations for cashmere production performance, H1H3:GGCC is preponderant combination for milk production performance. It may be a molecular marker for future selection and breeding. [ABSTRACT FROM AUTHOR]
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- 2023
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8. Transcriptomics analysis of cashmere fineness functional genes.
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Qin, Yuting, Xu, Yanan, Zhang, Yu, Gu, Ming, Cai, Weidong, Bai, Zhixian, Zhang, Xinjiang, Chen, Rui, Sun, Yinggang, Wu, Yanzhi, and Wang, Zeying
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GENETIC regulation , *CASHMERE , *GENE expression , *GOAT breeds , *GRAFT rejection - Abstract
Liaoning cashmere goat (LCG) is a famous cashmere goat breed in China. Cashmere fineness, as an important index to evaluate cashmere quality, is also one of the problems to be improved for Liaoning cashmere goats. Transcriptome studies all mRNA transcribed by a specific tissue or cell in a certain period. It is a key link in the study of gene expression regulation. It plays an important role in the analysis of biological growth and disease. Transcriptome is spatio-temporal specific, that is, gene expression varies in different tissues or at different times. Three coarser and three fine LCG skin samples were sequenced by RNA-seq technology, and a total of 427 differentially expressed genes were obtained, including 291 up-regulated genes and 136 down-regulated genes. In the experiment, we screened out 16 genes that had significant differences in the expression of coarse and fine cashmere of Liaoning cashmere goats, so it was inferred that these 16 genes might have regulatory effects on cashmere fineness. Moreover, GO gene set enrichment analysis revealed that differential genes mainly consist of immune response, MHC protein complex, Heme binding and other pathways. KEGG analysis showed that transplant-versus-host disease and allograft rejection were the main pathways of differential genes. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Association analysis for SNPs of KRT26 and TCHH genes with cashmere production performance, body measurement traits and milk production traits in Liaoning cashmere goats.
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Xu, Yanan, Zhang, Xinjiang, Hui, Taiyu, Sun, Jiaming, Cai, Weidong, Lin, Guangyu, Wang, Lingling, Dou, Xingtang, Wang, Zhanhong, Han, Di, Wang, Jiaming, Zhang, Yu, Qin, Yuting, Gu, Ming, Bai, Zhixian, Sun, Yinggang, Wu, Yanzhi, Chen, Rui, and Wang, Zeying
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MILK yield , *SINGLE nucleotide polymorphisms , *CASHMERE , *HAPLOTYPES , *GENETIC polymorphisms - Abstract
Cashmere fineness is getting thicker, which is one of the key problems in cashmere breeding, however, there have been no systematic studies on the molecular regulation of cashmere fineness. The aim of this study was to investigate the relationship between KRT26 and TCHH gene polymorphism and production performance in Liaoning cashmere goats (LCG). The potential single nucleotide polymorphisms (SNPs) of LCG were detected by sequence alignment and PCR-Seq polymorphism of KRT26 and TCHH genes and analyzed the effect of SNPs on production performance by SPSS software. Two SNPs sites (A559T and A6839G) of two genes were detected. The AA genotype of KRT26 A559T locus was the dominant genotype. AG and GG at TCHH A6839G locus were the dominant genotypes. AAAA was the dominant haplotype combination. The results showed that KRT26 and TCHH genes were associated with cashmere fineness of LCG, and A559T (AA) and A6839G (GG) genotypes were the preferred marker genotypes for cashmere fineness, which provided more theoretical basis for further research on cashmere fineness. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Metabolomic Analysis and MRM Verification of Coarse and Fine Skin Tissues of Liaoning Cashmere Goat.
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Xu, Yanan, Cai, Weidong, Chen, Rui, Zhang, Xinjiang, Bai, Zhixian, Zhang, Yu, Qin, Yuting, Gu, Ming, Sun, Yinggang, Wu, Yanzhi, and Wang, Zeying
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CHOLESTEROL metabolism , *METABOLOMICS , *CASHMERE , *GOATS , *LIQUID chromatography-mass spectrometry , *BILE acids - Abstract
One of the critical elements in evaluating the quality of cashmere is its fineness, but we still know little about how it is regulated at the metabolic level. In this paper, we use UHPLC–MS/MS detection and analysis technology to compare the difference in metabolites between coarse cashmere (CT_LCG) and fine cashmere (FT_LCG) skin of Liaoning cashmere goats. According to the data, under positive mode four metabolites were significantly up-regulated and seven were significantly down-regulated. In negative mode, seven metabolites were significantly up-regulated and fourteen metabolites were significantly down-regulated. The two groups' most significant metabolites, Gly–Phe and taurochenodeoxycholate, may be crucial in controlling cashmere's growth, development, and fineness. In addition, we enriched six KEGG pathways, of which cholesterol metabolism, primary bile acid biosynthesis, and bile secretion were enriched in positive and negative modes. These findings offer a new research idea for further study into the critical elements influencing cashmere's fineness. [ABSTRACT FROM AUTHOR]
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- 2022
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11. Selection of Cashmere Fineness Functional Genes by Translatomics.
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Zhang, Yu, Zhang, Dongyun, Xu, Yanan, Qin, Yuting, Gu, Ming, Cai, Weidong, Bai, Zhixian, Zhang, Xinjiang, Chen, Rui, Sun, Yingang, Wu, Yanzhi, and Wang, Zeying
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CASHMERE ,GENOME-wide association studies ,GENES ,NUCLEOTIDE sequencing - Abstract
Cashmere fineness is an important index to evaluate cashmere quality. Liaoning Cashmere Goat (LCG) has a large cashmere production and long cashmere fiber, but its fineness is not ideal. Therefore, it is important to find genes involved in cashmere fineness that can be used in future endeavors aiming to improve this phenotype. With the continuous advancement of research, the regulation of cashmere fineness has made new developments through high-throughput sequencing and genome-wide association analysis. It has been found that translatomics can identify genes associated with phenotypic traits. Through translatomic analysis, the skin tissue of LCG sample groups differing in cashmere fineness was sequenced by Ribo-seq. With these data, we identified 529 differentially expressed genes between the sample groups among the 27197 expressed genes. From these, 343 genes were upregulated in the fine LCG group in relation to the coarse LCG group, and 186 were downregulated in the same relationship. Through GO enrichment analysis and KEGG enrichment analysis of differential genes, the biological functions and pathways of differential genes can be found. In the GO enrichment analysis, 491 genes were significantly enriched, and the functional region was mainly in the extracellular region. In the KEGG enrichment analysis, the enrichment of the human papillomavirus infection pathway was seen the most. We found that the COL6A5 gene may affect cashmere fineness. [ABSTRACT FROM AUTHOR]
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- 2022
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12. Single-Cell Sequencing Reveals Differential Cell Types in Skin Tissues of Liaoning Cashmere Goats and Key Genes Related Potentially to the Fineness of Cashmere Fiber.
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Wang, Zeying, Wang, Yanru, Hui, Taiyu, Chen, Rui, Xu, Yanan, Zhang, Yu, Tian, He, Wang, Wei, Cong, Yuyan, Guo, Suping, Zhu, Yanxu, Zhang, Xinghui, Guo, Dan, Bai, Man, Fan, Yixing, Yue, Chang, Bai, Zhixian, Sun, Jiaming, Cai, Weidong, and Zhang, Xinjiang
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HAIR follicles ,CASHMERE ,GENE expression profiling ,GOATS ,GENE regulatory networks ,RNA sequencing - Abstract
Cashmere fineness is one of the important factors determining cashmere quality; however, our understanding of the regulation of cashmere fineness at the cellular level is limited. Here, we used single-cell RNA sequencing and computational models to identify 13 skin cell types in Liaoning cashmere goats. We also analyzed the molecular changes in the development process by cell trajectory analysis and revealed the maturation process in the gene expression profile in Liaoning cashmere goats. Weighted gene co-expression network analysis explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2 , a marker gene of SDPCs, was selected for immunofluorescence (IF) and Western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results show different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify and prove the differential expression in the coarse and fine types of Liaoning cashmere goats. Therefore, the CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat SDPCs; our research provides new insights into the mechanism of cashmere growth and fineness regulation by cells. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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