1. TCR-induced, PKC-θ-mediated NF-κB activation is regulated by a caspase-8-caspase-9-caspase-3 cascade.
- Author
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Yixia Zhao, Minxiang Lei, Zhaoyuan Wang, Guilin Qiao, Tianlun Yang, and Jian Zhang
- Subjects
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T cell receptors , *PROTEIN kinase C , *NF-kappa B , *CASPASES , *APOPTOSIS , *PHOSPHORYLATION - Abstract
It has been documented that caspase-8, a central player in apoptosis, is also crucial for TCR-mediated NF-κB activation. However, whether other caspases are also involved this process is unknown. In this report, we showed that in addition to caspase-8, caspase-9 is required for TCR-mediated NF-κB activation. Caspase-9 induces activation of PKC-θ, phosphorylation of Bcl10 and NF-κB activation in a caspase-3-dependent manner, but it appears that Bcl10 phosphorylation is uncoupled from NF-κB activation. Furthermore, caspase-8 lies upstream of caspase-9 during T cell activation. Therefore, TCR ligation elicits a caspase cascade involving caspase-8, caspase-9 and caspase-3 which initiates PKC-θ-dependent pathway leading to NF-κB activation and PKC-θ-independent Bcl10 phosphorylation which limits NF-kB activity. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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