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Unlabelled: The aim of the study was to analyze the physicochemical synthesized complex 3 [(1,3- thiazol -2- ylimino) methyl)]-4H- chromene -4 -one with tetrachloroplatinate(II) dipotassium and determination peroxidase activity and glutathione (GPX) in red blood cells of cancer patients and healthy subjects., Materials and Methods: Tests were carried out with the approval of the Bioethics Committee No. RNN/260/08/KB. Blood was collected into tubes with anticoagulant (heparin lithium). Determination of glutathione peroxidase activity was performed by methods of Little and O'Brien in 20 person groups hospitalized at the Department of General and Colorectal Surgery Veterans General Hospital in Łódź., Results: The study was an increase of activity in the control without the compound and after the introduction of the complex relative to the treatment groups. In healthy subjects, without the use of glutathione peroxidase complex averaged 73.25 ± 23.88 U / g Hb after application of the compound corresponds to the reference group 81.01 ± 25.94 U / g Hb. In contrast, in patients without the use of the complex activity amounted to 42.85 ± 27.49 U / g Hb. In the study group, which uses synthesized complex GPX activity corresponds to 67.72 ± 13.44 U / g Hb., Conclusions: The obtained results underline that the introduction of significant blood antioxidant complex research has a significant impact on the results of the determinations. Statistically significant (p < 0.05) difference occurred in both test and no relation to the administration of the complex in relation to the control of 1. 2.

Unlabelled: Coxarthrosis used to be referred to as the "doctors' cross" pointing to the difficulties of the treatment. Degenerative processes in the bones usually concern large joints: knee joints in 25-40% of cases, similarly in case of hip joints. Because of the role the joints play, the arthrosis may result in physical limitations or disability. The aim of the study was to evaluate the activity of catalase and superoxide dismutase (CuZn-SOD) in erythrocytes in patients suffering from the coxarthrosis--as the studied group and in healthy people--as the control group., Materials and Methods: Determining the activity of catalase and superoxide dismutase in the group of 17 patients hospitalized because of coxarthrosis., Results: In the study about 90% increase of the activity of the enzymes was determined in the studied group, in which the chemical compound 3{(2-aminothiazolyl)methylene}4H-1-benzopyran-4-one was added to the blood, in comparison with the control group., Conclusion: The results of the test on catalase and superoxide dismutase clearly indicate that the free radicals participate in the whole course of coxarthrosis.

Unlabelled: Coxarthrosis results in physical limitations (hypokinesis) and a necessity to change one's life style to the one that does not require much movement. Physical inactivity leads rapidly to metabolic processes inhibition. The processes of protein, carbohydrate and lipid catabolism intensify. Abnormal metabolism of polyunsaturated fatty acids occurs, which consequently leads to the development of reactive oxygen species (ROS) and to the acceleration of lipid peroxidation. Therefore, it is well-founded to seek for a new group of compounds demonstrating interesting biochemical properties. Among such already known compounds are chromone, azole and flavone derivatives, known for their oxidative properties. The aim of the study was to assess the antioxidant enzymes: catalase (CAT) and glutathione peroxidase (GPX) in erythrocytes in 25 patients suffering from coxarthrosis in comparison with healthy persons., Materials and Methods: The activity of catalase was determined in erythrocytes using the method of Beers and Sizer at 240 nm on the Beckman spectrophotometer. The activity of glutathione peroxidase was estimated using the method of Little and O'Brien at 412 nm on the Beckman spectrophotometer. The research was conducted with the permit of the Bioethics Committee No RNN/260/08/KB dated 20.05.2008. Blood for the tests was taken from periulnar vein to anticoagulant tubes (EDTA). The group consisted of 25 patients hospitalized in the Trauma and Orthopaedic Ward of the Hospital in Łódź., Results: The results of the tests on erythrocytes in the patients with physical limitations reveal the increase of glutathione peroxidase and catalase activity by about 50% in all patients, who were administered the dichlorobis (N1-dichloromethyl-3,5-dimethylpyrazole, copper (II) compound in comparison to the control group, where the foregoing compound was not used., Conclusion: The research results significantly emphasise that adding of new chemical compounds to blood in investigation of oxidative properties has a considerable influence on the activity of the selected enzymes (catalase and glutathione peroxidase) in comparison to the control group. The research reveals an increase of the activity of all antioxidant enzymes, where newly synthesized chemical compounds were used. The results of antioxidative capacity tests confirmed that complex compounds with Cu (II) ions have a significant influence on the antioxidative status. The studies on the foregoing enzymes also indicate that overproduced free radicals participate in the whole course of the coxarthrosis.

Unlabelled: Chemotherapy is an important field of clinical medicine and pharmacology Chemotherapy is the main method of treating the neoplasm. It involves treating the neoplastic disease with the use of natural or synthetic anticancer drugs commonly known as the cytostatics. The cancer therapy involving the cytostatics is a difficult and not always effective process, which requires taking into consideration the mechanisms of action, pharmacokinetics and the dosage schemas. The complexity of the chemotherapy is influenced by: still incomplete knowledge of causes of neoplasm formation, slight biological differences between the cancer cells and the regular cells, very small selectivity of cytostatics' action, narrow therapeutic index of the anticancer drugs, high toxicity for healthy cells and unsatisfactory anticancer activity. The aim of the study was to obtain the complex compounds of copper (II), and especially of dinitrate (V) di (3,4, 5-trimethyl-N1-pyrazol-kappaN2) copper (II), and its subsequent testing for pro- and antioxidative activity in people suffering from neoplastic diseases of the digestive tract (colorectal and gastric carcinoma). Material and methods. The activity of catalase was determined in erythrocytes patients suffering from colorectal and gastric carcinoma and in control group (hernia, chronic gastric ulcer disease and haemorrhoids) using the method of Beers and Sizer at 240 nm on the Beckman spectrophotometer., Results: The results of the test on catalase in patients suffering from colorectal and gastric carcinoma indicate that the addition to the blood of the complex compound of Cu(II) significantly influences the activity of the enzyme in comparison with the control group in which the substance tested was not used. In the tests a marked statistical difference was observed between the studied and the control group., Conclusion: The results reveal that chemical compounds, that is, dinitrate (V) di (3,4,5-trimethyl-N1-pyrazol-kappaN2) copper (II), has a significant influence on the activity of catalase, the antioxidant enzyme.