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2 results on '"M. Di Fenza"'

1. Subfertility in bulls carrying a nonsense mutation in transmembrane protein 95 is due to failure to interact with the oocyte vestments.

Author

Fernandez-Fuertes B, Laguna-Barraza R, Fernandez-Gonzalez R, Gutierrez-Adan A, Blanco-Fernandez A, O'Doherty AM, Di Fenza M, Kelly AK, Kölle S, and Lonergan P

Subjects
Animals, Female, Male, Membrane Proteins genetics, Mutation, Sperm-Ovum Interactions genetics, Cattle genetics, Cattle Diseases genetics, Infertility, Male genetics, Membrane Proteins metabolism, Sperm-Ovum Interactions physiology, Spermatozoa physiology
Abstract

In a recent genome-wide association study, 40 Fleckvieh bulls with exceptionally poor fertility were found to be homozygous for a nonsense mutation in the transmembrane protein 95 (TMEM95) encoding gene. Ejaculates from these individuals exhibited normal sperm concentration, morphology, viability, and motility. However, only 1.7% of inseminations resulted in pregnancies. The aim of this study was to examine the effect of this mutation in TMEM95 on bovine sperm function in vitro. Sperm from homozygous (mt/mt) males had lower in vitro fertility than sperm from wild-type (wt/wt) or heterozygous (wt/mt) bulls (P < 0.01). In addition, early embryo division was affected in the mt/mt group (P < 0.01). This translated into a lower (P < 0.01) blastocyst rate at day 8. Fluorescent staining revealed that TMEM95 is lost after the acrosome reaction. This led us to hypothesize that TMEM95 might be involved in events that lead to sperm-oocyte interaction. After fertilization, a lower number (P < 0.01) of sperm from mt/mt bulls bound to the zona pellucida (ZP). Sperm from mt/mt bulls were also less able to penetrate oocytes with no ZP (P< 0.01). However, when sperm from these animals were injected into mouse oocytes, they could decondense as successfully as sperm from wt/wt bulls. No differences between genotypes were observed in the ability of sperm to retain motility in an ex vivo oviduct, or in the percentage of sperm exhibiting markers for capacitation and acrosomal reaction. These results suggest that fertilization failure in mt/mt bulls is due to the inability of their sperm to interact with the oocyte vestments., (© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2017
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2. Lipopolysaccharide (LPS) disrupts particle transport, cilia function and sperm motility in an ex vivooviduct model

Author

Sabine Kölle, Alan M. O'Doherty, and M. Di Fenza

Subjects
Lipopolysaccharides, Male, 0301 basic medicine, medicine.medical_specialty, animal structures, LPS, Lipopolysaccharide, Reproductive disorders, Immunology, Motility, Oviducts, In Vitro Techniques, Biology, Article, Andrology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Human fertilization, Internal medicine, medicine, Animals, Cilia, Sperm motility, Inflammation, Sexually transmitted diseases, STDs, Microscopy, 030219 obstetrics & reproductive medicine, Multidisciplinary, Gene Expression Profiling, Reproduction, Embryogenesis, Sperm, Oxidative Stress, 030104 developmental biology, Endocrinology, Fertility, chemistry, Sperm Motility, TLR4, Oviduct, Cattle, Female, Bacterial infection
Abstract

The oviduct functions in the transportation of gametes to the site of fertilization (the ampulla) and is the site of early embryonic development. Alterations of this early developmental environment, such as the presence of sexually transmitted pathogens, may affect oviduct function leading to reduced fertilization rates and contribute to compromised embryonic development. In this study, sperm interactions, particle transport speed (PTS) and cilia beat frequency (CBF) in the ampulla following exposure to lipopolysaccharide (LPS), a constituent of the sexually transmitted pathogens Chlamydia trachomatis and Chlamydia abortus, was investigated. Three complementary experiments were performed to analyse; (1) bound sperm motility and cilia function (2) transport velocity in the oviduct and (3) the expression of genes related to immune function and inflammatory response (CASP3, CD14, MYD88, TLR4 and TRAF6). The motility of bound sperm was significantly lower in ampullae that were exposed to LPS. CBF and PTS significantly increased after treatment with LPS for 2 hours. Finally, gene expression analysis revealed that CASP3 and CD14 were significantly upregulated and TLR4 trended towards increased expression following treatment with LPS. These findings provide an insight on the impact of LPS on the oviduct sperm interaction, and have implications for both male and female fertility.

Published
2016

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