1. Immune microenvironment profiling of gastrointestinal stromal tumors (GIST) shows gene expression patterns associated to immune checkpoint inhibitors response
- Author
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Maria A. Pantaleo, Giuseppe Tarantino, Claudio Agostinelli, Milena Urbini, Margherita Nannini, Maristella Saponara, Chiara Castelli, Silvia Stacchiotti, Elena Fumagalli, Lidia Gatto, Donatella Santini, Antonio De Leo, Teresa Marafioti, Ayse Akarca, Elena Sabattini, Andrea Pession, Andrea Ardizzoni, Valentina Indio, and Annalisa Astolfi more...
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gastrointestinal stromal tumor ,gist ,tumor-infiltrating lymphocytes ,til ,ifn-γ signaling pathway ,cd4+ t cells ,cd8+ t cells ,m2 macrophages ,pd-l1 expression ,immunotherapy ,checkpoint inhibitor ,imatinib ,Immunologic diseases. Allergy ,RC581-607 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Few studies were conducted investigating the immunological profiles in gastrointestinal stromal tumors (GIST). Adaptive and innate immune cells are present in the tumor microenvironment, indicating GIST as inflamed tumors. In addition, murine models suggested a potential interaction between immune components and imatinib. In this retrospective study, the GIST immunological profile was investigated through in silico analysis and immunohistochemistry (IHC), exploring the basis for immunotherapy approaches. Gene expression profiles (GEP) from 31 KIT/PDGFRA-mutant GIST were analyzed to evaluate the tumor microenvironment and immunotherapy predictive signatures such as the expanded IFN-γ-induced immune signature (EIIS) and the T-cell-inflamed signature (TIS). GEP and IHC supported the presence of immune infiltrate in GIST, with dominance of CD4+ and CD8+ T cells and M2 macrophages showing a remarkable similarity with melanoma microenvironment. The EIIS genes were expressed in most of GIST samples and positively correlated with PD-L1 abundance (p more...
- Published
- 2019
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