1. Usefulness of IL-21, IL-7, and IL-15 conditioned media for expansion of antigen-specific CD8+ T cells from healthy donor-PBMCs suitable for immunotherapy.
- Author
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Chamucero-Millares JA, Bernal-Estévez DA, and Parra-López CA
- Subjects
- Adult, CD8-Positive T-Lymphocytes metabolism, Cell Differentiation drug effects, Culture Media, Conditioned chemistry, Cytotoxicity, Immunologic, Epitopes, T-Lymphocyte immunology, Female, Humans, Immunologic Memory drug effects, Immunotherapy, Adoptive methods, Interleukins metabolism, Lymphocyte Activation drug effects, Lymphocyte Activation immunology, Male, Signal Transduction, T-Lymphocytes, Cytotoxic cytology, T-Lymphocytes, Cytotoxic drug effects, T-Lymphocytes, Cytotoxic immunology, CD8-Positive T-Lymphocytes cytology, CD8-Positive T-Lymphocytes immunology, Cell Culture Techniques methods, Culture Media, Conditioned pharmacology, Interleukins pharmacology
- Abstract
Clonal anergy and depletion of antigen-specific CD8+ T cells are characteristics of immunosuppressed patients such as cancer and post-transplant patients. This has promoted translational research on the adoptive transfer of T cells to restore the antigen-specific cellular immunity in these patients. In the present work, we compared the capability of PBMCs and two types of mature monocyte-derived DCs (moDCs) to prime and to expand ex-vivo antigen-specific CD8+ T cells using culture conditioned media supplemented with IL-7, IL-15, and IL-21. The data obtained suggest that protocols involving moDCs are as efficient as PBMCs-based cultures in expanding antigen-specific CD8+ T cell to ELA and CMV model epitopes. These three gamma common chain cytokines promote the expansion of naïve-like and central memory CD8+ T cells in PBMCs-based cultures and the expansion of effector memory T cells when moDCs were used. Our results provide new insights into the use of media supplemented with IL-7, IL-15, and IL-21 for the in-vitro expansion of early-differentiated antigen-specific CD8+ T cells for immunotherapy purposes., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2021
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