1. Aberrantly Expressed Genes and miRNAs in Slow Transit Constipation Based on RNA-Seq Analysis.
- Author
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Zhao, Shipeng, Chen, Qiang, Kang, Xianwu, Kong, Bin, and Wang, Zhuo
- Subjects
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GENETICS of bacterial diseases , *CONSTIPATION , *CELL adhesion molecules , *GENE expression , *HISTOCOMPATIBILITY antigens , *GENETIC mutation , *RNA , *STAPHYLOCOCCUS aureus , *GENE expression profiling , *SEQUENCE analysis , *GENETICS - Abstract
Background. This study aims to identify the key genes and miRNAs in slow transit constipation (STC). Methods. MRNA and miRNA expression profiling were obtained. Differentially expressed genes (DEGs) and miRNAs were identified followed by the regulatory network construction. Functional annotation analysis and protein-protein interaction (PPI) network were conducted. The electronic validation was performed. Results. Hsa-miR-2116-3p, hsa-miR-3622a-5p, hsa-miR-424-5p, and hsa-miR-1273-3p covered most DEGs. HLA-DRB1, HLA-DRB5, C3, and ICAM were significantly involved in staphylococcus aureus infection. The PPI network generated several hub proteins including ZBTB16, FBN1, CCNF, and CDK1. Electronic validation of HLA-DRB1, PTGDR, MKI67, BIRC5, CCNF, and CDK1 was consistent with the RNA-sequencing analysis. Conclusion. Our study might be helpful in understanding the pathology of STC at the molecular level. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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