1. The MAP Kinase MPK4 Is Required for Cytokinesis in Arabidopsis thaliana[W]
- Author
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Ken Kosetsu, Takashi Soyano, Sachihiro Matsunaga, Yasunori Machida, Heribert Hirt, Hirofumi Nakagami, Michiko Sasabe, Yuji Takahashi, Jean Colcombet, Nagoya University, Osaka University [Osaka], University of Vienna [Vienna], Unité de recherche en génomique végétale (URGV), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Program for the Promotion of Basic Research Activities for Innovative Biosciences, Scientific Research on Priority Areas [19060003], Ministry of Education, Culture, Sports, Science and Technology of Japan, and Global COE program
- Subjects
0106 biological sciences ,[SDV]Life Sciences [q-bio] ,Recombinant Fusion Proteins ,Arabidopsis ,PROTEIN ,BREFELDIN-A ,Plant Science ,MAP Kinase Kinase 6 ,Septin ,01 natural sciences ,Plant Roots ,PATHWAY ,03 medical and health sciences ,PHRAGMOPLAST ,Arabidopsis thaliana ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Animals ,KINESIN ,Tissue Distribution ,Kinase activity ,Research Articles ,030304 developmental biology ,Cytokinesis ,Mitogen-Activated Protein Kinase Kinases ,0303 health sciences ,biology ,Kinase ,CELL-PLATE FORMATION ,Arabidopsis Proteins ,TOBACCO CELLS ,Cell Biology ,Cell plate ,biology.organism_classification ,PLANT CYTOKINESIS ,GENE ,Cell biology ,Phenotype ,Mitogen-activated protein kinase ,DEFENSE RESPONSES ,Mutation ,biology.protein ,Mitogen-Activated Protein Kinases ,010606 plant biology & botany ,Signal Transduction - Abstract
Cytokinesis in plants is achieved by the formation of the cell plate. A pathway that includes mitogen-activated protein (MAP) kinase kinase kinase and MAP kinase kinase (MAPKK) plays a key role in the control of plant cytokinesis. We show here that a MAP kinase, MPK4, is required for the formation of the cell plate in Arabidopsis thaliana. Single mutations in MPK4 caused dwarfism and characteristic defects in cytokinesis, such as immature cell plates, which became much more prominent upon introduction of a mutation in MKK6/ANQ, the MAPKK for cytokinesis, into mpk4. MKK6/ANQ strongly activated MPK4 in protoplasts, and kinase activity of MPK4 was detected in wild-type tissues that contained dividing cells but not in mkk6/anq mutants. Fluorescent protein–fused MPK4 localized to the expanding cell plates in cells of root tips. Expansion of the cell plates in mpk4 root tips appeared to be retarded. The level of MPK11 transcripts was markedly elevated in mpk4 plants, and defects in the mpk4 mpk11 double mutant with respect to growth and cytokinesis were more severe than in the corresponding single mutants. These results indicate that MPK4 is the downstream target of MKK6/ANQ in the regulation of cytokinesis in Arabidopsis and that MPK11 is also involved in cytokinesis.
- Published
- 2010
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