1. Nuclease Rsn from Rhizopus stolonifer: specificity and mode of action
- Author
-
V Shankar and E.S Rangarajan
- Subjects
Buffaloes ,Stereochemistry ,Biophysics ,Biochemistry ,Substrate Specificity ,Endonuclease ,chemistry.chemical_compound ,Hydrolysis ,Animals ,Magnesium ,Mode of action ,Molecular Biology ,Manganese ,Nuclease ,biology ,Phosphoric Diester Hydrolases ,Cobalt ,DNA ,Cell Biology ,Endonucleases ,biology.organism_classification ,Intestines ,Liver ,chemistry ,Time course ,biology.protein ,Tetra ,Rhizopus stolonifer ,Cattle ,Rhizopus ,Spleen ,Snake Venoms - Abstract
Nuclease Rsn from Rhizopus stolonifer catalyzes the hydrolysis of ss- and dsDNA in a ratio of approximately 2:1. Time course of 3 ′ and 5 ′ terminal analysis of the hydrolytic products of ss- and dsDNA showed that nuclease Rsn does not show any strict base preference and cleaves DNA in a non-specific manner. Moreover, separation of the hydrolytic products of ss- and dsDNA in the presence of Mg 2+ , Mn 2+ or Co 2+ showed the predominance of tetra-, tri-, and dinucleotides followed by mononucleotides, suggesting an endo mode of action.
- Published
- 2004
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