1. Small RNAs guide histone methylation in Arabidopsis embryos
- Author
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Rowan P. Herridge, Jonathan Cahn, Robert A. Martienssen, Daniel Grimanelli, and Jean-Sébastien Parent
- Subjects
Small RNA ,Heterochromatin ,Arabidopsis ,Epigenesis, Genetic ,Histones ,Research Communication ,03 medical and health sciences ,Histone H3 ,0302 clinical medicine ,Histone methylation ,Genetics ,Arabidopsis thaliana ,Gene Silencing ,030304 developmental biology ,0303 health sciences ,biology ,Arabidopsis Proteins ,DNA Methylation ,biology.organism_classification ,Cell biology ,RNA, Plant ,030220 oncology & carcinogenesis ,Seeds ,DNA methylation ,Reprogramming ,Developmental Biology - Abstract
Epigenetic reprogramming occurs during gametogenesis as well as during embryogenesis to reset the genome for early development. In flowering plants, many heterochromatic marks are maintained in sperm, but asymmetric DNA methylation is mostly lost. Asymmetric DNA methylation is dependent on small RNA but the re-establishment of silencing in embryo is not well understood. Here we demonstrate that small RNAs direct the histone H3 lysine 9 dimethylation during Arabidopsis thaliana embryonic development, together with asymmetric DNA methylation. This de novo silencing mechanism depends on the catalytic domain of SUVH9, a Su(Var)3-9 homolog thought to be catalytically inactive.
- Published
- 2021
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