1. Identification of BOIP, a novel cDNA highly expressed during spermatogenesis that encodes a protein interacting with the orange domain of the hairy-related transcription factor HRT1/Hey1 in Xenopus and mouse.
- Author
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Van Wayenbergh R, Taelman V, Pichon B, Fischer A, Kricha S, Gessler M, Christophe D, and Bellefroid EJ
- Subjects
- Amino Acid Sequence, Animals, Basic Helix-Loop-Helix Transcription Factors, Cell Cycle Proteins metabolism, Cloning, Molecular, Conserved Sequence, Embryo, Mammalian metabolism, Embryo, Nonmammalian, Gene Library, HeLa Cells, Humans, Immunohistochemistry, In Situ Hybridization, Luciferases metabolism, Male, Membrane Proteins metabolism, Mice, Molecular Sequence Data, Nuclear Proteins biosynthesis, Nuclear Proteins genetics, Plasmids metabolism, Precipitin Tests, Protein Binding, Protein Structure, Tertiary, RNA metabolism, RNA, Messenger metabolism, Receptors, Notch, Ribonucleases metabolism, Sequence Homology, Amino Acid, Signal Transduction, Testis metabolism, Transfection, Two-Hybrid System Techniques, Xenopus, Carrier Proteins biosynthesis, Carrier Proteins genetics, Cell Cycle Proteins chemistry, DNA, Complementary metabolism, Gene Expression Regulation, Developmental, Spermatogenesis, Spermatozoa metabolism
- Abstract
Hairy-related transcription factor (HRT/Hey) genes encode a novel subfamily of basic helix-loop-helix (bHLH) transcription factors related to the Drosophila hairy and Enhancer-of-split (E(spl)) and the mammalian HES proteins that function as downstream mediators of Notch signaling. Using the yeast two-hybrid approach, a previously uncharacterized protein was identified in Xenopus that interacts with XHRT1 (originally referred to as bc8), one member of the HRT/Hey subclass. This protein is evolutionarily conserved in chordates. It binds to sequences adjacent to the bHLH domain of XHRT1 known as the Orange domain and has been named bc8 Orange interacting protein (BOIP). BOIP shows a rather uniform subcellular localization and is recruited to the nucleus upon binding to XHRT1. In Xenopus, XBOIP mRNA is detected by RNase protection analysis throughout embryogenesis. In the adult, the strongest expression is detected in testis. In the mouse, high levels of BOIP mRNA are also found in adult testis. No expression is detected in the embryo and in any of the other adult organs tested. In situ hybridization revealed that BOIP transcripts were detected almost exclusively in round spermatids and that this expression overlaps with that of Hey1 (HRT1), which is expressed throughout spermatogenesis. In view of the importance of the Orange domain for HRT/Hey function, the newly identified BOIP proteins may serve as regulators specifically of HRT1/Hey1 activity., (Copyright 2003 Wiley-Liss, Inc.)
- Published
- 2003
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