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Start Over Author "Li, Dang" Topic cell differentiation
5 results on '"Li, Dang"'

1. Runx2 acts downstream of C/EBPβ to regulate the differentiation of uterine stromal cells in mice.

Author

Guo CH, Yue ZP, Bai ZK, Li DD, Yang ZQ, and Guo B

Subjects
Animals, Core Binding Factor Alpha 1 Subunit genetics, Cyclic AMP metabolism, Cyclooxygenase 2 metabolism, Decidua cytology, Female, In Situ Hybridization, Male, Matrix Metalloproteinase 9 metabolism, Mice, Pregnancy, Stromal Cells cytology, Stromal Cells metabolism, Vascular Endothelial Growth Factor A metabolism, CCAAT-Enhancer-Binding Protein-beta metabolism, Cell Differentiation, Core Binding Factor Alpha 1 Subunit metabolism, Uterus cytology
Abstract

Although Runx2 is involved in the regulation of cellular differentiation, its physiological roles in the differentiation of uterine stromal cells during decidualization still remain unknown. The aim of this study was to examine the expression, regulation and function of Runx2 in mouse uterus during decidualization. The results showed that Runx2 was highly expressed in the decidua and oil-induced decidualized cells. In the uterine stromal cells, recombinant human Runx2 (rRunx2) could induce the expression of Prl8a2 and Prl3c1 which are two well-known differentiation markers for decidualization, while inhibition of Runx2 with specific siRNA reduced their expression. Further study found that rRunx2 could improve the expression of Prl8a2 and Prl3c1 in the C/EBPβ siRNA-transfected stromal cells. In the stromal cells, cAMP analogue 8-Br-cAMP could induce the expression of Runx2. Moreover, the induction was blocked by PKA inhibitor H89. Simultaneously, attenuation of C/EBPβ with siRNA could also reduce the cAMP-induced Runx2 expression. Furthermore, siRNA-mediated silencing of Runx2 expression alleviated the effects of cAMP on the differentiation of stromal cells. Runx2 might act downstream of C/EBPβ to regulate the expression of Cox-2, Vegf and Mmp9 in the uterine stromal cells. Collectively, Runx2 may play an important role during mouse decidualization.

Published
2016
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2. Single-cell RNA-seq reveals the diversity of trophoblast subtypes and patterns of differentiation in the human placenta

Author

Cheng Zhu, Hongmei Wang, Xiaoyin Lu, Hai-Yan Lin, James C. Cross, Ya-Wei Liu, Xiaoying Fan, Yan-Li Dang, Rui Wang, Huiying Wang, and Hao Ge

Subjects
0301 basic medicine, Peptide Hormones, Placenta, Cell, Gene Expression, Biology, Article, 03 medical and health sciences, Pregnancy, medicine, Humans, Molecular Biology, Gene, reproductive and urinary physiology, Fetus, Cytotrophoblast, Base Sequence, Sequence Analysis, RNA, Mesenchymal stem cell, Placentation, Trophoblast, Cell Differentiation, Mesenchymal Stem Cells, Cell Biology, Trophoblasts, Cell biology, Pregnancy Trimester, First, 030104 developmental biology, medicine.anatomical_structure, Pregnancy Trimester, Second, embryonic structures, Female, Single-Cell Analysis
Abstract

The placenta is crucial for a successful pregnancy and the health of both the fetus and the pregnant woman. However, how the human trophoblast lineage is regulated, including the categorization of the placental cell subtypes is poorly understood. Here we performed single-cell RNA sequencing (RNA-seq) on sorted placental cells from first- and second-trimester human placentas. New subtypes of cells of the known cytotrophoblast cells (CTBs), extravillous trophoblast cells (EVTs), Hofbauer cells, and mesenchymal stromal cells were identified and cell-type-specific gene signatures were defined. Functionally, this study revealed many previously unknown functions of the human placenta. Notably, 102 polypeptide hormone genes were found to be expressed by various subtypes of placental cells, which suggests a complex and significant role of these hormones in regulating fetal growth and adaptations of maternal physiology to pregnancy. These results document human placental trophoblast differentiation at single-cell resolution and thus advance our understanding of human placentation during the early stage of pregnancy.

Published
2018

3. PLAC8, a new marker for human interstitial extravillous trophoblast cells, promotes their invasion and migration

Author

Cheng Zhu, Wenxiang Meng, Ya-Wei Liu, Wen-Lin Chang, Haibin Wang, Xing Huang, H.E. Xu, Li-Qun Xue, Xiangxiang Jiang, Yan-Li Dang, Hai-Yan Lin, Yan-Ling Wang, and Hongmei Wang

Subjects
0301 basic medicine, rac1 GTP-Binding Protein, Cellular differentiation, Human Development, Placenta, PLAC8, RAC1, CDC42, Biology, 03 medical and health sciences, Invasion and migration, 0302 clinical medicine, Pre-Eclampsia, Cell Movement, Pregnancy, medicine, Decidua, Humans, RNA, Small Interfering, cdc42 GTP-Binding Protein, Molecular Biology, reproductive and urinary physiology, Monomeric GTP-Binding Proteins, 030219 obstetrics & reproductive medicine, Trophoblast, Proteins, Extravillous trophoblast cells, Cell Differentiation, Marker, Preeclampsia, Coculture Techniques, Cell biology, Trophoblasts, Up-Regulation, 030104 developmental biology, medicine.anatomical_structure, Cdc42 GTP-Binding Protein, Case-Control Studies, Gene Knockdown Techniques, embryonic structures, Female, Chorionic Villi, Filopodia, Biomarkers, Developmental Biology
Abstract

Proper differentiation of trophoblast cells in the human placenta is a prerequisite for a successful pregnancy, and dysregulation of this process may lead to malignant pregnancy outcomes, such as preeclampsia. Finding specific markers for different types of trophoblast cells is essential for understanding trophoblast differentiation. Here, we report that placenta-specific protein 8 (PLAC8) is specifically expressed in the interstitial extravillous trophoblast cells (iEVTs) on the fetomaternal interface. Using model systems, including placental villi-decidua co-culture, iEVTs induction by using primary trophoblast cells or explants, etc., we found that PLAC8 promotes invasion and migration of iEVTs. Mechanistically, time-lapse imaging, GTPase activity assay, co-immunoprecipitation and RNA-seq studies show that PLAC8 increases the Cdc42 and Rac1 activities, and further induces the formation of filopodia at the leading edge of the migratory trophoblast cells. More interestingly, PLAC8 is significantly upregulated under hypoxia and expression of PLAC8 is higher in iEVTs from preeclamptic placentas when compared with those from the normal control placentas. Together, PLAC8 is a new marker for iEVTs and plays an important role in promoting trophoblast invasion and migration., Highlighted Article: Oxygen tension-dependent expression of placenta-specific protein 8 positively regulates trophoblast invasion and migration partially through upregulating the activation of Rac1 and Cdc42.

Published
2018

4. Expression, regulation and function of Hmgn3 during decidualization in mice.

Author

Li, Dang-Dang, Guo, Chuan-Hui, Yue, Liang, Duan, Cui-Cui, Yang, Zhan-Qing, Cao, Hang, Guo, Bin, and Yue, Zhan-Peng

Subjects
*GENE expression, *CELL differentiation, *LABORATORY mice, *STROMAL cells, *EPITHELIAL cells, *SMALL interfering RNA
Abstract

Although Hmgn3 is involved in the regulation of development and cellular differentiation, its physiological roles on decidualization are still unknown. Here we showed that Hmgn3 was highly expressed in the decidua and decidualizing stromal cells. Overexpression of Hmgn3 variants, Hmgn3a or Hmgn3b, enhanced the expression of decidualization markers Prl8a2 and Prl3c1, whereas inhibition of Hmgn3 reduced their expression. Hmgn3 could mediate the effects of Hoxa10 and cAMP on the expression of Prl8a2 and Prl3c1. Further study found that Hmgn3 directed the process of decidualization through influencing the expression of Hand2. Progesterone could induce the expression of Hmgn3 in the ovariectomized mouse uterus, uterine epithelial cells and stromal cells. Knockdown of Hoxa10 with siRNA alleviated the induction of progesterone and cAMP on Hmgn3 expression. Simultaneously, siRNA-mediated down-regulation of Hmgn3 in the uterine stromal cells could attenuate the effects of progesterone, cAMP and Hoxa10 on the expression of Hand2. Collectively, Hmgn3 may play an important role during mouse decidualization. [ABSTRACT FROM AUTHOR]

Published
2015
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5. Effects of PTHrP on chondrocytes of sika deer antler.

Author

Guo, Bin, Wang, Shou-Tang, Duan, Cui-Cui, Li, Dang-Dang, Tian, Xue-Chao, Wang, Qu-Yuan, and Yue, Zhan-Peng

Subjects
CARTILAGE cells, SIKA deer, PARATHYROID hormone-related protein, CELL differentiation, IN situ hybridization, TRANSCRIPTION factors, GENE expression, CELL proliferation
Abstract

Parathyroid-hormone-related peptide (PTHrP) is an important regulator of chondrocyte differentiation in growth plates but little is known about its role in deer antler cartilage. The aim of the present study was to use the deer antler as a model to determine the possible role of PTHrP in regulating chondrocyte differentiation of deer antler. PTHrP and its receptor PTH1R mRNA were highly expressed in the perichondrium and cartilage of sika deer antler, as shown by in situ hybridization. Chondrocytes of deer antler were identified by toluidine blue staining of glycosaminoglycan and immunocytochemical staining of type II collagen (Col II). Treatment with PTHrP (1-34) reduced the expression of prehypertrophic chondrocyte marker Col IX and hypertrophic chondrocyte marker Col X. In order to confirm the mechanism of action of PTHrP, we initially examined the expression of cyclin D1, Bcl-2 and runt-related transcription factor 2 (Runx2) in sika deer antler by in situ hybridization and found that cyclin D1, Runx2 and Bcl-2 mRNA were also expressed in antler chondrocytes. Exogenous PTHrP induced the expression of cyclin D1 and Bcl-2 mRNA by various signalling pathways, whereas it inhibited Runx2 expression through PKA, p38MAPK, MEK and PI3K signalling pathways. Thus, PTHrP might promote the proliferation of antler chondrocytes and prevent their differentiation; it might furthermore influence the growth and development of sika deer antler. [ABSTRACT FROM AUTHOR]

Published
2013
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