1. ERF115 controls root quiescent center cell division and stem cell replenishment.
- Author
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Heyman J, Cools T, Vandenbussche F, Heyndrickx KS, Van Leene J, Vercauteren I, Vanderauwera S, Vandepoele K, De Jaeger G, Van Der Straeten D, and De Veylder L
- Subjects
- Anaphase-Promoting Complex-Cyclosome metabolism, Arabidopsis Proteins genetics, Cell Cycle genetics, Cell Cycle physiology, Cell Cycle Proteins metabolism, Cell Division genetics, Mitosis genetics, Mitosis physiology, Peptide Hormones genetics, Peptide Hormones metabolism, Proteolysis, Signal Transduction, Stem Cell Niche, Transcription Factors genetics, Arabidopsis cytology, Arabidopsis growth & development, Arabidopsis Proteins metabolism, Cell Division physiology, Plant Roots cytology, Plant Roots growth & development, Stem Cells physiology, Transcription Factors metabolism
- Abstract
The quiescent center (QC) plays an essential role during root development by creating a microenvironment that preserves the stem cell fate of its surrounding cells. Despite being surrounded by highly mitotic active cells, QC cells self-renew at a low proliferation rate. Here, we identified the ERF115 transcription factor as a rate-limiting factor of QC cell division, acting as a transcriptional activator of the phytosulfokine PSK5 peptide hormone. ERF115 marks QC cell division but is restrained through proteolysis by the APC/C(CCS52A2) ubiquitin ligase, whereas QC proliferation is driven by brassinosteroid-dependent ERF115 expression. Together, these two antagonistic mechanisms delimit ERF115 activity, which is called upon when surrounding stem cells are damaged, revealing a cell cycle regulatory mechanism accounting for stem cell niche longevity.
- Published
- 2013
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