1. FB-15 inhibits MGC-803 cells growth by regulating energy metabolism.
- Author
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Deng, Xiangping, Pi, Yiyuan, Li, Zhongli, Xiong, Runde, Liu, Juan, Zhao, Jingduo, Xie, Zhizhong, Lei, Xiaoyong, and Tang, Guotao
- Subjects
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CELL growth , *ENERGY metabolism , *CELL migration , *MOLECULAR docking , *STOMACH cancer , *TUBULINS - Abstract
In this study, we scrutinized the anticancer effects of FB-15 on human gastric carcinoma MGC-803 cells in vitro and vivo , and its preliminary effect on tubulin and HIF-1α. We confirmed that FB-15 not only inhibited the proliferation of a large number of cells in a concentration and time-dependent manner but also inhibited proliferation of a single cell to form clones. FB-15 manifested little cytotoxicity for normal stomach cells GES-1. The flow cytometry analysis displayed that FB-15 induced apoptosis MGC-803 cells and mainly arrested cells in the S phase in a concentration-dependent manner. The results of the wound healing assay indicated that FB-15 suppressed cell migration. Furthermore, the western blotting showed that FB-15 down-regulated the expression of β3-tubulin and HIF-1α, consistent with Immunohistochemical assay. The binding modes of FB-15 with tubulin were clarified by molecular docking. FB-15 significantly suppressed the growth of MGC-803 gastric cancer tumors. The inhibitory effect of FB-15 on tumor growth was superior to 5-Fu. Taken together, these results provided evidence for FB-15 to be used as an effective anticancer drug candidate for gastric cancer. • FB-15 is a flavonoid benzimidazole derivative. • FB-15 down-regulates tubulin and glycolysis-related protein. • FB-15 shows potent anticancer activity against MGC-803 cells in vitro / vivo. • The binding mode of FB-15 with tubulin is clarified by molecular docking. • FB-15 might regulate energy metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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