1. DNA walker for signal amplification in living cells.
- Author
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Yuan, Aijiao, Xiao, Hang, Yang, Fan, Hao, Huifang, Wang, Xiaoyan, Li, Jinhua, Jin, Minghua, Zhao, Qiang, Sha, Rui, Deng, Zhaojia, and Peng, Hanyong
- Subjects
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DNA , *DNA nanotechnology , *CELL imaging , *DNA folding , *CELL physiology , *BIOMOLECULES , *DEOXYRIBOZYMES , *MOLECULAR motor proteins - Abstract
The rapid development of DNA nanotechnology enables the programmable operation of advanced DNA walkers, mimicking the function of protein motor in living organisms. The walking systems inherently require four main components for construction, including motor, track, energy, and cargo. They are able to perform specific functions autonomously following a predesigned trajectory with nanometer precision. Research has focused on improving the three key features of DNA walkers: progressivity, speed, and directionality. The new DNA nano techniques have exhibited the powerful ability for signal amplification during their operation. This review will cover the breakthrough design and principle of walking systems, and highlight the recent application of DNA walkers for signal amplification in living cells. The engineering DNA walkers have great potential for diverse applications, such as imaging intracellular molecules, modulating cellular functions and facilitating drug delivery. • Mechanisms behind those milestones during the evolution of artificial engineered DNA walker have been extensively discussed. • Continuous innovation of DNA walker with novel elements improves their performance in movement and functionality. • Engineering DNA walker enables in situ cellular imaging of disease-related biomolecules with spatiotemporal information. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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