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The detailed mechanism and the extent of pH/SO2 changes during apoptosis remain unknown. The developed sensor NPCF for SO2 and pH dual detection illustrates that SO2 can reduce the inflammation caused by LPS and the acidification of the environment. The levels of SO2 and pH change during carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced apoptosis. [ABSTRACT FROM AUTHOR]

• It is the first QM-based AIE fluorescent probe for ONOO−. • QM-ONOO− showed bigger Stokes shift (190 nm) than the reported. • QM-ONOO− possessed rapid response, and low detection limit (27.5 nM). • The exogenous and endogenous ONOO− imaging in MCF-7 cells demonstrated its value of practical tool. Peroxynitrite (ONOO−), as one type of reactive nitrogen species (RNS) , is closely related to multiple pathophysiological processes. Visualization of ONOO− in living system is essential for further study of its physiological functions. However, most existing ONOO− fluorescent probes continuously display the aggregation-caused quenching (ACQ). In present research, a new activatable AIEgen fluorescent probe QM-ONOO− for ONOO− recognition and imaging was proposed. Activation of the probe by ONOO−, induced aggregation of QM−OH. This sensor showed fast response time (<4 min) and high sensitivity (DL, 27.5 nM), large Stock's shift (190 nm), long emission wavelength. Furthermore, the outcomes indicate that QM-ONOO− as an activatable AIEgen fluorescent sensor was robust chemical tool to detect endogenous peroxynitrite in living biological system. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • A new N-oxide based and turn on fluorescent probe for Fe2+ was developed. • The probe possessed a high selectivity for Fe2+ among various biologically relevant species and fast rapid response. • Monitoring Fe2+ with TPM fluorescence imaging for the first time. Abstract Iron is an essential element for human body, involved in important biochemical processes in the body, especially in biological oxidation. Misregulation of iron levels in the cell is concerned with serious diseases. Here, a novel two photon and turn on NIR-fluorescent probe based ICT for detection of Fe2+ is first reported. The probe exhibited brilliant selectivity and excellent sensitivity (DL = 4.5 μM), rapid response (15 min) and "naked-eye colorimetric sensor" for monitoring of Fe2+. More importantly, the probe was successfully applied to real-time monitor Fe2+ in living cell through two-photon microscopy fluorescence imaging with excitation 680 nm. [ABSTRACT FROM AUTHOR]

A ER-targetable probe R1 for HOCl was synthesized. R1 shows excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. • A new ER-targetable ICT-based fluorescent probe R1 for HClO was developed. • Probe have rapid response (30 s), big Stock's shift and a wide range of pH(7–13). • The imaging in EC1 cells demonstrated its value of practical application. The endoplasmic reticulum is the largest organelle in the cell. Studies have shown that the concentration of HClO is related to many diseases. A new type of probe R1 has positioned to the endoplasmic reticulum. The probe shows good positioning effect, excellent selectivity and high sensitivity, excellent anti-interference, and can quickly reach the response platform. The obvious color change can be used for naked eye observation. Due to its low cytotoxicity, probe R1 was successfully used for EC1 cells imaging under a laser scanning confocal microscope. [ABSTRACT FROM AUTHOR]