1. Fluorescence-guided surgery of a highly-metastatic variant of human triple-negative breast cancer targeted with a cancer-specific GFP adenovirus prevents recurrence
- Author
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Yano, Shuya, Takehara, Kiyoto, Miwa, Shinji, Kishimoto, Hiroyuki, Tazawa, Hiroshi, Urata, Yasuo, Kagawa, Shunsuke, Bouvet, Michael, Fujiwara, Toshiyoshi, and Hoffman, Robert M
- Subjects
Biomedical and Clinical Sciences ,Oncology and Carcinogenesis ,Cancer ,Breast Cancer ,Adenoviridae ,Animals ,Cell Line ,Tumor ,Cells ,Cultured ,Disease Models ,Animal ,Female ,Gene Expression ,Genetic Vectors ,Green Fluorescent Proteins ,Humans ,Mice ,Microscopy ,Fluorescence ,Optical Imaging ,Recurrence ,Surgery ,Computer-Assisted ,Transduction ,Genetic ,Triple Negative Breast Neoplasms ,Xenograft Model Antitumor Assays ,breast cancer ,triple negative ,nude mice ,orthotopic ,fluorescence-guided surgery ,telomerase dependent ,adenovirus ,GFP/RFP ,survival ,Oncology and carcinogenesis - Abstract
We have previously developed a genetically-engineered GFP-expressing telomerase-dependent adenovirus, OBP-401, which can selectively illuminate cancer cells. In the present report, we demonstrate that targeting a triple-negative high-invasive human breast cancer, orthotopically-growing in nude mice, with OBP-401 enables curative fluorescence-guided surgery (FGS). OBP-401 enabled complete resection and prevented local recurrence and greatly inhibited lymph-node metastasis due to the ability of the virus to selectively label and subsequently kill cancer cells. In contrast, residual breast cancer cells become more aggressive after bright (white)-light surgery (BLS). OBP-401-based FGS also improved the overall survival compared with conventional BLS. Thus, metastasis from a highly-aggressive triple-negative breast cancer can be prevented by FGS in a clinically-relevant mouse model.
- Published
- 2016