Author: "De Marchis, Laura" / Topic: cell lines - Searchworks@Jio Institute Digital Library Search Results

Author: Manzari, Vittorio, Gismondi, Angela, Barillari, Giovanni, Morrone, Stefania, Modesti, Andrea, Albonici, Loredana, De Marchis, Laura, Fazio, Vito, Gradilone, Angela, Zani, Massimo, Frati, Luigi, and Santoni, Angela
Published: 1987

Author: Traicoff, June L., De Marchis, Laura, Ginsburg, Britten L., Zamora, Rodolfo E., Khattar, Nada H., Blanch, Vincent J., Plummer, Sarah, Bargo, Sharon E., Templeton, Dennis J., Casey, Graham, and Kaetzel, Charlotte S.
Subjects: *COLON tumors, *COLON cancer, *CELL lines, *CELL culture, *TUMORS, *MESSENGER RNA, *ADENOMA, *IMMUNOGLOBULINS
Abstract: The human cell lines VACO-235 and VACO-411 constitute a novel in vitro model of colon adenoma to carcinoma progression. By differential display RT-PCR we identified a transcript that is expressed in the parental nontumorigenic adenoma line (VACO-235E), but is not expressed in the tumorigenic daughter (VACO-235L) or granddaughter (VACO-411) lines. This cDNA represents a previously uncharacterized portion of the 3'UTR of human PIGR. Human PIGR mRNA was found to be highly expressed in normal colon epithelium, but was decreased in 6 of 8 colon tumors and was negligible in 8 of 10 colon tumor cell lines. We sequenced the entire 1.8 kb 3'UTR of human PIGR, and found it to contain multiple repetitive elements as well as elements that could affect the processing and stability of PIGR mRNA. We hypothesize that differential regulation of PIGR mRNA stability may contribute to its downregulation in colon cancer. Copyright ? 2003 National Science Council, ROC and S. Karger AG, Basel. [ABSTRACT FROM AUTHOR]
Published: 2003
Full Text: View/download PDF

Searchworks