1. Arsenoplatin-Ferritin Nanocage: Structure and Cytotoxicity.
- Author
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Ferraro, Giarita, Pratesi, Alessandro, Cirri, Damiano, Imbimbo, Paola, Maria Monti, Daria, Messori, Luigi, Merlino, Antonello, and Maki, Masatoshi
- Subjects
FERRITIN ,EMISSION spectroscopy ,ULTRAVIOLET-visible spectroscopy ,CANCER cells ,CELL lines - Abstract
Arsenoplatin-1 (AP-1), the prototype of a novel class of metallodrugs containing a PtAs(OH)
2 core, was encapsulated within the apoferritin (AFt) nanocage. UV-Vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy measurements confirmed metallodrug encapsulation and allowed us to determine the average amount of AP-1 trapped inside the cage. The X-ray structure of AP-1-encapsulated AFt was solved at 1.50 Å. Diffraction data revealed that an AP-1 fragment coordinates the side chain of a His residue. The biological activity of AP-1-loaded AFt was comparatively tested on a few representative cancer and non-cancer cell lines. Even though the presence of the cage reduces the overall cytotoxicity of AP-1, it improves its selectivity towards cancer cells. [ABSTRACT FROM AUTHOR]- Published
- 2021
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