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Tylophora indica (Burm f.) Merrill, belong to family Asclepiadaceae, is considered to be a natural remedy with high medicinal benefits. The objective of this work is to assess the metabolomic profile of T. indica leaves enriched in alkaloids, as well as to evaluate the in vitro cytotoxicity of these leaves using the MTT assay on human breast MCF-7 and liver HepG2 cancer cell lines. Dried leaves of T. indica were extracted by sonication, using methanol containing 2 % (v /v) of acetic acid and obtained fraction was characterized by HPTLC and UPLC-MS. The UPLC-MS study yielded a preliminary identification of 32 metabolites, with tylophorine, tylophorine B, tylophorinine, and tylophorinidine being the predominant metabolites. The cytotoxicity of the extract of T. indica was evaluated on HepG2 and MCF-7 cell lines, yielding inhibitory concentration (IC 50) values of 75.71 μg/mL and 69.60 μg/mL, respectively. Data suggested that the phytochemical screening clearly showed presence of numerous secondary metabolites with moderate cytotoxic efficacy. In conclusion, the future prospects of T. indica appear promising for the advancement of phytopharmaceutical-based anticancer medications, as well as for the design of contemporary pharmaceuticals in the field of cancer chemotherapy. [Display omitted] • The alkaloid-rich fraction of T. indica leaves comprises phenanthroindolizidine alkaloids and other secondary metabolites. • It has been observed that these alkaloids possess significant cytotoxic properties against MCF-7 and HepG2 cancer cell lines. • These compounds can be tested for their ability to selectively affect metabolic pathways in lab settings and living organisms. • The potential of T. indica for the development of phytopharmaceutical-based anticancer drugs is highly promising. • T. indica has great potential in the development of advanced pharmaceuticals for cancer chemotherapy. [ABSTRACT FROM AUTHOR]

Natural resources have been used as medicine for the treatment of many diseases. Algae have been used as both food and traditional medicine in many countries. Algae consist of several novel biologically active metabolites having biological activities such as anti-viral, antitumor and anti-inflammatory activities. The aim of present study is to evaluate the anti-cancer properties of different unique marine macro algae against three different cancer cell lines such as Hela, MCF-7 and HepG2 cell lines. Seven different species of marine algae named as MA1, MA2, MA3, MA4, MA5, MA6 and MA7 were collected from the coastal region surrounding Rameswaram in Tamilnadu. Different concentrations of the methanolic extracts of these algae were initially screened for cytotoxicity on the cell lines by MTT assay. One of the algal extracts (MA1) exhibited maximum cytotoxicity against all the tested cancer cell lines. The extract from this algal strain was taken up for purification by TLC using different solvent combinations. Further, anticancer activity was analysed by various in-vitro assays and purification of the bioactive compound by chromatography technique was performed. The Algal isolate exhibited great promise as potential candidate for purification and characterization studies. [ABSTRACT FROM AUTHOR]