1. The ERH gene regulates migration and invasion in 5637 and T24 bladder cancer cells
- Author
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Lin Hao, Han Xiaoxiao, Guang-hui Zang, Jie Wang, Jianjun Zhang, Zhiguo Zhang, Kun Pang, Longjun Cai, Rui Li, Bo Chen, Zhen-duo Shi, Conghui Han, Ying Liu, and Yang Dong
- Subjects
0301 basic medicine ,Cancer Research ,Cell ,Mice, Nude ,Cell Cycle Proteins ,urologic and male genital diseases ,lcsh:RC254-282 ,Small hairpin RNA ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Nude mouse ,ERH gene ,Cell Movement ,Cell Line, Tumor ,Gene expression ,Genetics ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Migration and invasion ,Gene knockdown ,Migration Assay ,biology ,Chemistry ,Gene Expression Profiling ,Bladder cancer ,Cell migration ,biology.organism_classification ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Gene expression profiling ,030104 developmental biology ,medicine.anatomical_structure ,Urinary Bladder Neoplasms ,Oncology ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Cancer research ,MYC gene ,Research Article ,Transcription Factors - Abstract
Background This study aimed to determine whether the enhancer of the rudimentary homolog (ERH) gene regulates cell migration and invasion in human bladder urothelial carcinoma (BUC) T24 cells and the underlying mechanism. Methods First, we knocked down ERH in BUC T24 and 5637 cells by shRNA and then used wound healing cell scratch migration assays, transwell cell migration assays, transwell cell invasion chamber experiments and nude mouse tail vein transfer assays to determine the migration and invasion ability after ERH was knocked down. Moreover, we used gene expression profiling chip analysis and further functional experiments to explore the possible mechanism through which ERH knockdown downregulated metastasis ability in T24 cells. Results Wound healing cell scratch migration assays, transwell cell migration assays, transwell cell invasion chamber experiments and nude mouse tail vein transfer assays all showed that the metastasis ability was significantly inhibited in human BUC T24 and 5637 cells with ERH knockdown. A gene expression profiling chip analysis in T24 cells showed that the MYC gene may be an important downstream target of the ERH gene, and the functional experiments showed that MYC is a functional target of ERH in BUC T24 cells. Conclusion ERH knockdown could inhibit the metastasis of BUC T24 cells in vitro and in vivo. This study further explored the mechanism of the ERH gene in the metastasis of the T24 human bladder cancer cell line and found that ERH may regulate MYC gene expression. The results of this research provide a basis for the clinical application of ERH as a potential target for BUC treatment. Electronic supplementary material The online version of this article (10.1186/s12885-019-5423-9) contains supplementary material, which is available to authorized users.
- Published
- 2019