1. A paraptosis-like cell death induced by δ-tocotrienol in human colon carcinoma SW620 cells is associated with the suppression of the Wnt signaling pathway.
- Author
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Zhang JS, Li DM, He N, Liu YH, Wang CH, Jiang SQ, Chen BQ, and Liu JR
- Subjects
- Apoptosis drug effects, Caspase 3 metabolism, Cell Line, Tumor, Colonic Neoplasms pathology, Dose-Response Relationship, Drug, Flow Cytometry, Humans, Mitochondria metabolism, Signal Transduction drug effects, Vitamin E administration & dosage, Vitamin E pharmacology, Wnt Proteins drug effects, Wnt1 Protein antagonists & inhibitors, Cell Death drug effects, Cell Proliferation drug effects, Colonic Neoplasms drug therapy, Vitamin E analogs & derivatives, Wnt Proteins antagonists & inhibitors
- Abstract
Tocotrienol is considered a beneficial effect agent on inhibition of tumor development. In this study, we focused on the effects of δ-tocotrienol and its possible mechanism on induction of death in human colon cancer SW620 cells. δ-Tocotrienol inhibited proliferation of SW620 cell in a dose-dependent manner. Our findings showed that δ-tocotrienol effectively induced paraptosis-like death in SW620 cells, correlated with the vacuolation that may be from welling and fusion of mitochondria and/or the endoplasmic reticulum (ER) as well as caspase-3 nonactivated. However, there were no changes in apoptosis based on flow cytometry analysis. Of being noted, δ-tocotrienol reduced the expression of β-catenin and wnt-1 proteins by about 50% at the highest dose (20μmol/L). δ-Tocotrienol also decreased cyclin D1, c-jun and MMP-7 protein levels in SW620 cells. Altogether, these data indicate that δ-tocotrienol induces paraptosis-like cell death, which is associated with the suppression of the Wnt signaling pathway. Thus, our findings may provide a novel application in treatment of human colon carcinoma., (Crown Copyright © 2011. Published by Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2011
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