1. Structure elucidation of arabinogalactoglucan isolated from Sedum sarmentosum Bunge and its inhibition on hepatocellular carcinoma cells in vitro.
- Author
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Zhang X, Bi C, Chen Q, Xu H, Shi H, and Li X
- Subjects
- Apoptosis drug effects, Arabinose analysis, Carcinoma, Hepatocellular pathology, Cell Cycle drug effects, Cell Line, Tumor, Galactose analysis, Humans, Liver Neoplasms pathology, Molecular Weight, Antineoplastic Agents chemistry, Carcinoma, Hepatocellular metabolism, Cell Proliferation drug effects, Glucans chemistry, Glucans pharmacology, Liver Neoplasms metabolism, Plant Extracts chemistry, Plant Extracts pharmacology, Sedum chemistry
- Abstract
Sedum sarmentosum Bunge (SS) is clinically used as Chinese medicine for hepatitis related diseases treatment. The purpose of this study was to explore the chemical structures of polysaccharides from this plant. A neutral polysaccharide (SSWP) was isolated and purified by ion-exchange chromatography and Superdex-75 column. The obtained SSWP was a homogenous one with a molecular weight of 21.5 kDa according to the high-performance gel permeation chromatography. The major monosaccharide composition of SSWP was arabinose, glucose and galactose in a molar ratio of 2.4:1:1.8. The methylation analysis showed that SSWP consists mainly of Araf-(1→, →5)-Araf-(1→, →3,5)-Araf-(1→, →4)-Galp-(1→, →4)-Glcp-(1→. The NMR result and enzymatic digestion data comprehensively indicated that SSWP was a novel arabinogalactoglucan-type structure. The anticancer assay in vitro exhibited that SSWP could effectively inhibit 48.9% of Huh-7 cells growth at 50 μg/mL and arrest cells at S-phase, and induce tumor cells apoptosis. Together, polysaccharide from S. sarmentosum Bunge could be a potential natural antitumor agent., Competing Interests: Declaration of competing interest All authors declare no conflicts of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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