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Urokinase‐type plasminogen activator receptor (uPAR) is a glycolipid‐anchored protein located on the cell surface that is implicated in the promotion of metastasis. New fluorescent probes for the detection of uPAR expression that feature a rapid "turn‐on" response are reported here. They consist of a donor‐π‐acceptor‐based fluorophore conjugated with a uPAR‐binding AE105 peptide. The resulting AE105‐coupled uPAR‐targeting probes are weakly emissive in aqueous buffer solutions; however, a fluorescence "turn‐on" signal is instantly triggered upon specific binding to uPAR (KD=63.2 nM for P1 and 49.5 nM for P2), which restricts the rotational deactivation of the fluorophore. Applications of the probes were demonstrated in the imaging of uPAR overexpressed on the membrane of cancer cell and in a cell‐based uPAR inhibitor assay. [ABSTRACT FROM AUTHOR]