1. Cyanidin-3-O-glucoside promotes progesterone secretion by improving cells viability and mitochondrial function in cadmium-sulfate-damaged R2C cells.
- Author
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Li X, Lu JL, Sun JX, Jiang XW, Li XS, Li Y, Jiao R, Tian LM, and Bai WB
- Subjects
- Animals, Cell Line, Leydig Cells enzymology, Leydig Cells metabolism, Male, Matrix Metalloproteinases metabolism, Phosphoproteins metabolism, Rats, Reactive Oxygen Species metabolism, Superoxide Dismutase metabolism, Up-Regulation drug effects, Anthocyanins pharmacology, Cadmium Compounds toxicity, Cell Survival drug effects, Glucosides pharmacology, Mitochondria drug effects, Progesterone metabolism, Sulfates toxicity
- Abstract
Cadmium (Cd) is a poisonous metal that is toxic for male reproduction. Cyanidin-3-O-glucoside (C3G) as typical anthocyanin benefits many organs. In this study, we investigated the protective effects and associated underlying mechanisms of C3G against the toxicity of Cd on male reproduction in rat Leydig cell line R2C cells. Cells were pre-protected with C3G (5-160 μmol/L) for 2 h and then treated with cadmium sulfate (CdSO
4 ) (10-160 μmol/L) for 24 h. The results showed that cytotoxicity, mitochondrial damage, superoxide dismutase 2 (SOD2), and overproduction of reactive oxygen species (ROS) in CdSO4 -treated R2C cells were significantly reduced with C3G pre-treatment. Moreover, C3G pre-treatment led to upregulated expression of steroidogenic acute regulatory (StAR) protein and progesterone production. Our study suggests that C3G may be a potential therapeutic agent against Cd-induced reproductive toxicity., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2019
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