1. Comparative Effects of Vitamin C, Curcumin, and Sambucus nigra Extract on Cell Viability and Cytokine Levels in Cigarette-exposed Lung Epithelium Cell Cultures.
- Author
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ÖZKAYA, Abdullah Eren, VARLI, Hanife Sevgi, TÜRKOĞLU, Nelisa Laçin, YABACI TAK, Ayşegül, and KARAKÖSE OKYALTIRIK, Fatmanur
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VITAMIN C , *TUMOR necrosis factors , *CELL survival , *CELL culture , *CURCUMIN , *CYTOKINES - Abstract
Introduction: Cigarettes contain harmful chemicals leading to diseases such as lung cancer and coronary artery diseases, causing deaths globally. Smoking depletes antioxidants crucial for immune function. Inflammation involving cytokines is a natural defensemechanism. Antioxidants such as Sambucus nigra, curcumin, and vitamin C reduce oxidative stress. However, higher doses of antioxidants may have a potential pro-inflammatory aspect, revealing a complex relationship between antioxidants and inflammation. Method: Human lung epithelium (BEAS-2b) cells were cultured in T25 flasks until 90% confluence at 37 °C. Cigarette smoke extract (CSE) was prepared. Tumor necrosis factor alpha (TNF-a) and interleukin-6 (IL-6) cytokine levels from CSE-stimulated cells were measured using ELISA kits. The effects of vitamin C, Sambucus nigra, and curcumin were tested through MTT analysis at varying concentrations. CSE-treated cells were supplemented with antioxidants, and cytokine levels were assessed using ELISA kits. Results: After 24 h of incubation with various concentrations of CSE, the highest concentration (resulting in the lowest viability) was selected for subsequent stages. BEAS-2b cells stimulated with CSE for 24 h showed no IL-6 stimulation, and TNF-a measured positive at 6.06 ng. Antioxidant exposure for 24, 48, and 72 h yielded varying viability percentages, with higher viability rates observed in the vitamin C groups. Seven groups were selected for cytokine level analysis, and IL-6 or TNF-a stimulation was not measured. Conclusion: The impact of substances on cells depends on dose and exposure duration. Higher vitamin C doses were correlated with lower cell viability, indicating potential pro-inflammatory effects. Sambucus nigra and curcumin consistently exhibited lower viability rates. Adjusting doses in future studies may clarify the healing effects. CSE uniquely increased TNF-a cytokine levels, revealing its inflammatory effects, whereas other substances suppressed inflammation, reducing cytokine levels. [ABSTRACT FROM AUTHOR]
- Published
- 2024