1. Surface-enhanced Raman spectroscopic chemical imaging reveals distribution of pectin and its co-localization with xyloglucan inside onion epidermal cell wall.
- Author
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He Q, Yang J, Zabotina OA, and Yu C
- Subjects
- Plant Epidermis metabolism, Plant Epidermis chemistry, Plant Epidermis cytology, Metal Nanoparticles chemistry, Silver chemistry, Xylans metabolism, Xylans analysis, Pectins metabolism, Pectins analysis, Glucans metabolism, Glucans analysis, Spectrum Analysis, Raman methods, Cell Wall chemistry, Cell Wall metabolism, Onions metabolism, Onions cytology, Onions chemistry
- Abstract
The primary plant cell wall is a complex matrix composed of interconnected polysaccharides including cellulose, hemicellulose, and pectin. Changes of this dynamic polysaccharide system play a critical role during plant cell development and differentiation. A better understanding of cell wall architectures can provide insight into the plant cell development. In this study, a Raman spectroscopic imaging approach was developed to visualize the distribution of plant cell wall polysaccharides. In this approach, Surface-enhanced Raman scattering (SERS through self-assembled silver nanoparticles) was combined with Raman labels (4-Aminothiophenol. 4ATP) and targeted enzymatic hydrolysis to improve the sensitivity, specificity, and throughput of the Raman imaging technique, and to reveal the distribution of pectin and its co-localization with xyloglucan inside onion epidermal cell (OEC) wall. This technique significantly decreased the required spectral acquisition time. The resulted Raman spectra showed a high Raman signal. The resulted Raman images successfully revealed and characterized the pectin distribution and its co-localization pattern with xyloglucan in OEC wall., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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