1. Serotyping of cell culture-adapted subgroup 2 human rotavirus strains by neutralization
- Author
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G. Gerna, Massimo Battaglia, N. Passarani, E Cattaneo, G Milenesi, and Elena Percivalle
- Subjects
Rotavirus ,Serotype ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Biology ,medicine.disease_cause ,Microbiology ,Neutralization ,Feces ,medicine ,Humans ,Serotyping ,Cells, Cultured ,Antiserum ,Gel electrophoresis ,Complement Fixation Tests ,Infant ,Complement fixation test ,Virology ,Gastroenteritis ,Infectious Diseases ,Immunization ,Parasitology ,Research Article - Abstract
Nine human rotavirus strains from stools of infants with gastroenteritis were serially propagated in MA-104 cell cultures. All strains were identified as subgroup 2 rotaviruses by RNA gel electrophoresis, complement fixation, and enzyme-linked immunosorbent assay. The human rotavirus strains were propagated for 15 to 20 passages and then used for immunization of guinea pigs and rabbits. Animal antisera were also raised against a subgroup 1 human strain purified from stools and against the cell culture-adapted Wa strain, a reference subgroup 2 rotavirus of human origin. Cross-neutralization studies revealed the existence of two distinct serotypes within the cell culture-adapted subgroup 2 human rotaviruses: strains related and unrelated to strain Wa were classified as serotypes 1 and 3, respectively. Results with convalescent-phase sera from infants with primary rotavirus infections confirmed the existence of two serotypes within subgroup 2, and the serotypes responsible for primary subgroup 2 infections could be determined on the basis of the neutralizing reactivity of convalescent sera.