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Your search keyword '"SEB, Staphylococcal enterotoxin B"' showing total 2 results
Start Over Descriptor "SEB, Staphylococcal enterotoxin B" Topic cellular immunity
2 results on '"SEB, Staphylococcal enterotoxin B"'

1. Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies

Author

Hanekom, Willem A., Hughes, Jane, Mavinkurve, Maushumi, Mendillo, Megan, Watkins, Marcia, Gamieldien, Hoyam, Gelderbloem, Sebastian J., Sidibana, Mzwandile, Mansoor, Nazma, Davids, Virginia, Murray, Rose Ann, Hawkridge, Anthony, Haslett, Patrick A.J., Ress, Stanley, Hussey, Gregory D., and Kaplan, Gilla

Subjects
*CYTOKINES, *CELLULAR immunity, *T cells, *LYMPHOCYTES
Abstract

We optimized a whole blood intracellular cytokine assay to quantitate the frequency of specific CD4+ and CD8+ T cells in small volumes of whole blood from infants from developing countries. The assay is performed in two steps. First, whole blood is stimulated in the presence of specific antigens for 6–18 h, ending with cryopreservation of fixed white cells. These stimulation steps were specifically adapted to be practical and reliable in a rural, developing country field setting. Later, in a more resourceful setting, interferon-gamma producing CD4+ or CD8+ T cells are detected by flow cytometry. The assay proved sensitive and specific for detecting mycobacteria-specific immunity 10 weeks after Bacillus Calmette-Guerin (BCG) vaccination of newborns from a rural field site. [Copyright &y& Elsevier]

Published
2004
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2. MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines

Author

Tamara Berthoud, Stephen Todryk, Susanna Dunachie, Helen A. Fletcher, and Adrian V. S. Hill

Subjects
Male, Cellular immunity, ESAT6, six-kDa early secreted antigenic target, T-Lymphocytes, STAT, signal transducers and activators of transcription, MVA, modified vaccinia virus Ankara, APC, antigen presenting cell, Chemokine CXCL9, ELISA, enzyme linked immunoSorbant assay, FP9, fowlpox strain FP9, BME, β-mercaptoethanol, Immunology and Allergy, M, MVA, Interferon gamma, Flow cytometry, Malaria, Falciparum, PPD, purified protein derivative, IFN-γ, RLT, RNA lysis buffer, Vaccines, Synthetic, HPRT, hypoxanthine phosphoribosyl transferase, medicine.diagnostic_test, Malaria vaccine, Reverse Transcriptase Polymerase Chain Reaction, ELISPOT, MIG, Middle Aged, MIG, monokine induced by gamma, TRAP, thrombospondin related adhesive protein, Monokine, P, PEV3A, ELISPOT, enzyme linked immuno SPOT, PBMC, peripheral blood mononuclear cells, CXCL9, F, FP9, Female, medicine.drug, Research Paper, Adult, CSP, circumsporozoite protein, Adolescent, SEB, staphylococcal enterotoxin B, Immunology, Plasmodium falciparum, Biology, JAK, janus kinase, Interferon-gamma, Young Adult, RT-PCR, reverse transcription polymerase chain reaction, stomatognathic system, RPMI, Roswell Park Memorial Institute media, Malaria Vaccines, medicine, Animals, Humans, RNA, Messenger, AMA1, apical membrane antigen 1, 7AAD, 7-amino-actinomycin D, Aged, CFP10, culture filtrate protein 10, DOC, day of challenge, Brefeldin A, IFN-γ, interferon gamma, CXCL9, CXC chemokine ligand 9, CMV, cytomegalovirus, EBV, Epstein Barr Virus, Virology, Malaria, stomatognathic diseases, PHA, phytohaemagglutinin, Vaccine, Ex vivo, ICS, intracellular staining, ME, multi-epitope string
Abstract

For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.

Published
2009

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