Search

Your search keyword '"Chu, Shilong"' showing total 3 results
Start Over Author "Chu, Shilong" Topic cellular reprogramming
3 results on '"Chu, Shilong"'

1. Induction of Pluripotent Stem Cells from Mouse Embryonic Fibroblasts by Jdp2-Jhdm1b-Mkk6-Glis1-Nanog-Essrb-Sall4.

Author

Wang B, Wu L, Li D, Liu Y, Guo J, Li C, Yao Y, Wang Y, Zhao G, Wang X, Fu M, Liu H, Cao S, Wu C, Yu S, Zhou C, Qin Y, Kuang J, Ming J, Chu S, Yang X, Zhu P, Pan G, Chen J, Liu J, and Pei D

Subjects
Animals, Cell Differentiation genetics, Cells, Cultured, Chimera genetics, DNA-Binding Proteins genetics, Embryonic Stem Cells cytology, Embryonic Stem Cells metabolism, Euchromatin genetics, Euchromatin metabolism, F-Box Proteins genetics, Fibroblasts cytology, Fibroblasts metabolism, Heterochromatin genetics, Heterochromatin metabolism, Induced Pluripotent Stem Cells metabolism, Jumonji Domain-Containing Histone Demethylases genetics, Kruppel-Like Factor 4, MAP Kinase Kinase 6 genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, Nanog Homeobox Protein genetics, RNA-Seq, Repressor Proteins genetics, Transcription Factors genetics, Cellular Reprogramming genetics, DNA-Binding Proteins metabolism, F-Box Proteins metabolism, Induced Pluripotent Stem Cells cytology, Jumonji Domain-Containing Histone Demethylases metabolism, MAP Kinase Kinase 6 metabolism, Nanog Homeobox Protein metabolism, Repressor Proteins metabolism, Transcription Factors metabolism
Abstract

Reprogramming somatic cells to pluripotency by Oct4, Sox2, Klf4, and Myc represent a paradigm for cell fate determination. Here, we report a combination of Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb, and Sall4 (7F) that reprogram mouse embryonic fibroblasts or MEFs to chimera competent induced pluripotent stem cells (iPSCs) efficiently. RNA sequencing (RNA-seq) and ATAC-seq reveal distinct mechanisms for 7F induction of pluripotency. Dropout experiments further reveal a highly cooperative process among 7F to dynamically close and open chromatin loci that encode a network of transcription factors to mediate reprogramming. These results establish an alternative paradigm for reprogramming that may be useful for analyzing cell fate control., (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2019
Full Text
View/download PDF

2. Resolving Cell Fate Decisions during Somatic Cell Reprogramming by Single-Cell RNA-Seq.

Author

Guo L, Lin L, Wang X, Gao M, Cao S, Mai Y, Wu F, Kuang J, Liu H, Yang J, Chu S, Song H, Li D, Liu Y, Wu K, Liu J, Wang J, Pan G, Hutchins AP, Liu J, Pei D, and Chen J

Subjects
Animals, Female, Gene Expression Regulation, Developmental, Induced Pluripotent Stem Cells metabolism, Interferon-gamma genetics, Interferon-gamma metabolism, Kruppel-Like Factor 4, Male, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Mouse Embryonic Stem Cells metabolism, Phenotype, Signal Transduction, Transcription Factors genetics, Transcription Factors metabolism, Cell Differentiation genetics, Cell Lineage genetics, Cellular Reprogramming genetics, Cellular Reprogramming Techniques, Induced Pluripotent Stem Cells physiology, Mouse Embryonic Stem Cells physiology, Sequence Analysis, RNA, Single-Cell Analysis
Abstract

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs), which is a highly heterogeneous process. Here we report the cell fate continuum during somatic cell reprogramming at single-cell resolution. We first develop SOT to analyze cell fate continuum from Oct4/Sox2/Klf4- or OSK-mediated reprogramming and show that cells bifurcate into two categories, reprogramming potential (RP) or non-reprogramming (NR). We further show that Klf4 contributes to Cd34+/Fxyd5+/Psca+ keratinocyte-like NR fate and that IFN-γ impedes the final transition to chimera-competent pluripotency along the RP cells. We analyze more than 150,000 single cells from both OSK and chemical reprograming and identify additional NR/RP bifurcation points. Our work reveals a generic bifurcation model for cell fate decisions during somatic cell reprogramming that may be applicable to other systems and inspire further improvements for reprogramming., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Published
2019
Full Text
View/download PDF

3. Chemical reprogramming of mouse embryonic and adult fibroblast into endoderm lineage.

Author

Cao S, Yu S, Chen Y, Wang X, Zhou C, Liu Y, Kuang J, Liu H, Li D, Ye J, Qin Y, Chu S, Wu L, Guo L, Li Y, Shu X, Chen J, Liu J, and Pei D

Subjects
Animals, Cell Differentiation, Cell Self Renewal, Cells, Cultured, Female, HMGB Proteins analysis, Hepatocytes cytology, Mice, Mice, Inbred C57BL, SOXF Transcription Factors analysis, Cellular Reprogramming drug effects, Endoderm cytology, Fibroblasts cytology, Fibroblasts drug effects, Stem Cells cytology
Abstract

We report here an approach to redirecting somatic cell fate under chemically defined conditions without transcription factors. We start by converting mouse embryonic fibroblasts to epithelial-like cells with chemicals and growth factors. Subsequent cell fate mapping reveals a robust induction of SOX17 in the resulting epithelial-like cells that can be further reprogrammed to endodermal progenitor cells. Interestingly, these cells can self-renew in vitro and further differentiate into albumin-producing hepatocytes that can rescue mice from acute liver injury. Our results demonstrate a rational approach to convert mouse embryonic fibroblasts to hepatocytes and suggest that this mechanism-driven approach may be generalized for other cells., (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.)

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results