1. Combined IL-12 Plasmid and Recombinant SjGST Enhance the Protective and Anti-pathology Effect of SjGST DNA Vaccine Against Schistosoma japonicum
- Author
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Ching Nan Lin, Kin Mu Lee, Po Ching Cheng, Tsung Fu Kang, and Shih Yi Peng
- Subjects
0301 basic medicine ,Male ,Physiology ,DNA vaccination ,Schistosoma japonicum ,law.invention ,Immunologic Adjuvants ,Mice ,Plasmid ,law ,Animal Cells ,Immune Physiology ,Medicine and Health Sciences ,Vaccines, DNA ,Public and Occupational Health ,Glutathione Transferase ,Innate Immune System ,Vaccines ,Recombinant Vaccines ,biology ,lcsh:Public aspects of medicine ,Helminth Proteins ,Vaccination and Immunization ,Interleukin-12 ,Vaccination ,Infectious Diseases ,Schistosomiasis japonica ,Recombinant DNA ,Interleukin 12 ,Granulomas ,Schistosoma ,Cytokines ,Female ,Cellular Types ,Research Article ,Plasmids ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Immune Cells ,Immunology ,Schistosomiasis ,03 medical and health sciences ,Antigen ,Helminths ,parasitic diseases ,medicine ,Animals ,Humans ,Public Health, Environmental and Occupational Health ,Organisms ,Biology and Life Sciences ,lcsh:RA1-1270 ,Cell Biology ,Molecular Development ,biology.organism_classification ,medicine.disease ,Virology ,Invertebrates ,Mice, Inbred C57BL ,030104 developmental biology ,Immune System ,Preventive Medicine ,Developmental Biology - Abstract
Schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. Development of a vaccine is thought to be the most effective way to control this disease. Recombinant 26-kDa glutathione S-transferase (rSjGST) has previously been reported to achieve a worm reduction rate of 42–44%. To improve the efficiency of the vaccine against Schistosoma japonicum, we immunized mice with a combination of pcDNA vector-encoded 26-kDa SjGST (pcDNA/SjGST), IL-12 expressing-plasmid (pIL-12), and rSjGST. Co-vaccination with pcDNA/SjGST, pIL-12, and rSjGST led to a reduction in worm burden, hepatic egg burden, and the size of liver tissue granulomas than that in the untreated infection controls. In addition, we detected high levels of specific IgG, IgG1, and IgG2a against the rSjGST antigen in infected mice vaccinated with this combination of pcDNA/SjGST, pIL-12, and rSjGST. Moreover, high expression levels of Th2 cytokines, including IL-4 and IL-10, were also detected in this group, without diminished levels of IL-12, INF-γ, and TNF-α cytokines that are related to parasite killing. In conclusion, we have developed a new vaccination regimen against S. japonicum infection and shown that co-immunization with pcDNA/SjGST vaccine, pIL-12, and rSjGST has significant anti-parasite, anti-hepatic egg and anti-pathology effects in mice. The efficacy of this vaccination method should be further validated in large animals such as water buffalo. This method may help to reduce the transmission of zoonotic schistosomiasis japonica., Author Summary Schistosomiasis continues to be a serious global public health problem that considered by World Health Organization (WHO). More than 200 million people are infected and cause 280 thousand deaths every year. Among, Schistosoma japonicum causes the most severe pathological damages and the slowest immune resistance manifestation. It is estimated there is considerable economic loss in China due to the infection of human and domestic animals. Therefore, development of a useful vaccine is thought to be an efficient strategy to control and prevent schistosome infection. In this study, we co-immunized mice with pcDNA/SjGST vaccine, pIL-12 and rSjGST to develop a new vaccination regimen against schistosomiasis japonica. And we found this regimen can induce both specific cellular and humoral responses to attain a balance between parasite elimination and prevention of pathological tissue injury. The new regimen produced significant anti-parasite, anti-hepatic egg, and anti-pathology effects. Our method of vaccination can be applied in large livestock, such as water buffalo or cow that may help to reduce the transmission of zoonotic schistosomiasis japonica.
- Published
- 2016