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1. Cell-free conversion of isopenicillin N into deacetoxycephalosporin C by Cephalosporium acremonium mutant M-0198.

Author

Baldwin JE, Keeping JW, Singh PD, and Vallejo CA

Subjects
Acremonium enzymology, Chromatography, High Pressure Liquid, Acremonium metabolism, Cephalosporins metabolism, Penicillins metabolism
Abstract

In a cell-free system prepared by lysis of protoplasts of Cephalosporium acremonium mutant M-0198, isopenicillin N was converted into a penicillinase-resistant material that behaved like deacetoxycephalosporin C on high-pressure liquid chromatography analysis. This activity was found to be unstable to storage at -80 degrees C; 70-80% of the activity was lost after 1 day.

Published
1981
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