1. Neurosteroidogenesis in oligodendrocytes and Purkinje neurones of cerebellar cortex of dogs.
- Author
-
Yarim M and Kabakci N
- Subjects
- 3-Hydroxysteroid Dehydrogenases immunology, Animals, Antibodies, Monoclonal, Cerebellar Cortex cytology, Cerebellar Cortex enzymology, Cytochrome P-450 Enzyme System immunology, Female, Immunohistochemistry veterinary, Male, Oligodendroglia enzymology, Progesterone biosynthesis, Progesterone physiology, Purkinje Cells enzymology, 3-Hydroxysteroid Dehydrogenases analysis, Cerebellar Cortex metabolism, Cytochrome P-450 Enzyme System analysis, Dogs metabolism, Myelin Proteins biosynthesis, Oligodendroglia metabolism, Purkinje Cells metabolism
- Abstract
The cerebellum is a steroidogenic organ that expresses steroidogenic enzymes and produces neurosteroids. Purkinje neurones appear to be the most active steroidogenic cells in the cerebellar cortex. These neurones express 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), P450 side-chain cleavage (P450scc), 17 alpha-hydroxylase/c17, 20lyase (P450c17), P450 aromatase (P450arom) and produce pregnenolone, progesterone, dehydroepiandrosterone, androstenedion, oestradion and oestrone. Oligodendrocytes are predominantly the producer of myeline protein. The oligodendrocytes were identified by immunohistochemistry using a monoclonal antibody against myeline 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), a myeline specific enzyme. In this study we have examined the distribution of 3 beta-HSD and CNPase by immunohistochemistry using monoclonal antibody in canine cerebellar cortex. The localization of oligodendrocytes within the cerebellar cortex was determined to be close to Purkinje neurones. This result suggests that endogenous progesterone synthesized de novo in the Purkinje neurone can promote myeline protein synthesis in oligodentrocytes.
- Published
- 2004
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