1. Pentylenetetrazol kindling induces cortical astrocytosis and increased expression of extracellular matrix molecules in mice.
- Author
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Ueno H, Suemitsu S, Murakami S, Kitamura N, Wani K, Takahashi Y, Matsumoto Y, Okamoto M, and Ishihara T
- Subjects
- Animals, Astrocytes drug effects, Astrocytes pathology, Cerebral Cortex drug effects, Cerebral Cortex pathology, Extracellular Matrix drug effects, Extracellular Matrix pathology, Gliosis chemically induced, Gliosis pathology, Hippocampus drug effects, Hippocampus metabolism, Hippocampus pathology, Kindling, Neurologic drug effects, Kindling, Neurologic pathology, Male, Mice, Mice, Inbred C57BL, Astrocytes metabolism, Cerebral Cortex metabolism, Extracellular Matrix metabolism, Gliosis metabolism, Kindling, Neurologic metabolism, Pentylenetetrazole toxicity
- Abstract
Although epilepsy is one of the most common chronic neurological disorders with a prevalence of approximately 1.0 %, the underlying pathophysiology remains to be elucidated. Understanding the molecular and cellular mechanisms involved in the development of epilepsy is important for the development of appropriate therapeutic strategy. In this study, we investigated the effects of status epilepticus on astrocytes, microglia, and extracellular matrix (ECM) molecules in the somatosensory cortex and piriform cortex of mice. Activation of astrocytes was observed in many cortices except the retrosplenial granular cortex after pentylenetetrazol (PTZ)-induced kindling in mice. Activated astrocytes in the cortex were found in layers 1-3 but not in layers 4-6. In the somatosensory and piriform cortices, no change was observed in the number of parvalbumin (PV)-positive neurons and PV-positive neurons covered with perineuronal nets. However, the amount of ECM in the extracellular space increased. The expression of VGLUT1- and GAD67-positive synapses also increased. Thus, in the PTZ-kindling epilepsy mice model, an increase in the number of ECM molecules and activation of astrocytes were observed in the somatosensory cortex and piriform cortex. These results indicate that PTZ-induced seizures affect not only the hippocampus but also other cortical areas. Our study findings may help to develop new therapeutic approaches to prevent seizures or their sequelae., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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