1. DPA714 PET Imaging Shows That Inflammation of the Choroid Plexus Is Active in Chronic-Phase Intracerebral Hemorrhage.
- Author
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Yao S, Gao Z, Fang W, Fu Y, Xue Q, Lai T, Shangguan H, Sun W, Lin Y, Lin F, and Kang D
- Subjects
- Humans, Mice, Animals, Inflammation diagnostic imaging, Inflammation metabolism, Positron-Emission Tomography methods, Choroid Plexus diagnostic imaging, Choroid Plexus metabolism, Cerebral Hemorrhage diagnostic imaging
- Abstract
Purpose: Our aims were to investigate the presence of choroid plexus (CP) inflammation in chronic-phase intracerebral hemorrhage (ICH) patients and to characterize any inflammatory cells in the CP., Patients and Methods: An in vivo 18 F-DPA714 PET study was undertaken in 22 chronic-phase ICH patients who were admitted to the First Affiliated Hospital of Fujian Medical University or Tianjin Medical University General Hospital from April 2017 to June 2020. Ten control participants with nonhemorrhagic central nervous system diseases were included. Choroid plexus 18 F-DPA714 uptake was calculated as the average SUVR. To aid the interpretation of the 18 F-DPA714 uptake results at the CP level, Cy5-DPA714 in vivo imaging and immunofluorescence staining were used to show the presence of CP inflammation in an ICH mouse model during the chronic phase (14 weeks after ICH). Then immunofluorescence staining against translocator protein and other specific biomarkers was used to characterize the cells present in the inflamed CP of ICH mice in the chronic phase., Results: PET imaging showed that CP DPA714 SUVRs in chronic-phase ICH patients were higher than in controls (mean CP SUVR ± SD; ICH group: 1.05 ± 0.35; control group: 0.81 ± 0.21; P = 0.006). Immunofluorescence staining of the CP in ICH model mice identified a population of CD45 + immune cells, peripheral monocyte-derived CD14 + cells, CD68 + phagocytes, and CD11b + resident microglia/macrophages expressing translocator protein, possibly contributing to the increased 18 F-DPA714 uptake., Conclusions: Our study shows that CP DPA714 uptake in chronic-phase ICH patients was higher than that of participants with nonhemorrhagic central nervous system diseases, which means that CP inflammation is still active in chronic-phase ICH patients., Competing Interests: Conflicts of interest and sources of funding: The authors declared no conflicts of interest. The study was supported by the National Natural Science Foundation of China (grant no. 82171327, 82171982, and 82371466), the Stroke Prevention and Treatment Project of the National Health Commission–Research and Popularization of Appropriate Intervention Technology for the Stroke High Risk Group in China (GN2018R002), the Fujian Science and Technology Innovation Joint Fund Project (2019Y9118), the Technology Platform Construction Project of Fujian Province (2020Y2003 and 2021Y2001), the Major Scientific Research Specialty Project of Fujian Province (2022ZD01003), and the Natural Science Foundation of Fujian Province (2023J01590 and 2023J06031). The funders had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the article; and decision to submit the article for publication., (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2024
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