1. Protein conformational changes and protonation dynamics probed by a single shot using quantum-cascade-laser-based IR spectroscopy.
- Author
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Schubert, Luiz, Langner, Pit, Ehrenberg, David, Lorenz-Fonfria, Victor A., and Heberle, Joachim
- Subjects
PROTON transfer reactions ,CHEMICAL amplification ,SPECTROMETRY ,MEMBRANE proteins ,QUANTUM cascade lasers ,BACTERIORHODOPSIN - Abstract
Mid-IR spectroscopy is a powerful and label-free technique to investigate protein reactions. In this study, we use quantum-cascade-laser-based dual-comb spectroscopy to probe protein conformational changes and protonation events by a single-shot experiment. By using a well-characterized membrane protein, bacteriorhodopsin, we provide a comparison between dual-comb spectroscopy and our homebuilt tunable quantum cascade laser (QCL)-based scanning spectrometer as tools to monitor irreversible reactions with high time resolution. In conclusion, QCL-based infrared spectroscopy is demonstrated to be feasible for tracing functionally relevant protein structural changes and proton translocations by single-shot experiments. Thus, we envisage a bright future for applications of this technology for monitoring the kinetics of irreversible reactions as in (bio-)chemical transformations. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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