1. Isolation of a novel lutein-protein complex from Chlorella vulgaris and its functional properties.
- Author
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Cai X, Huang Q, and Wang S
- Subjects
- Algal Proteins chemistry, Algal Proteins isolation & purification, Animals, Animals, Outbred Strains, Antioxidants administration & dosage, Antioxidants chemistry, Antioxidants isolation & purification, Biological Products chemistry, Biological Products isolation & purification, Biomarkers blood, Biomarkers metabolism, Carbon Tetrachloride Poisoning metabolism, Carbon Tetrachloride Poisoning pathology, Carbon Tetrachloride Poisoning physiopathology, Carbon Tetrachloride Poisoning prevention & control, Chemical and Drug Induced Liver Injury metabolism, Chemical and Drug Induced Liver Injury pathology, Chemical and Drug Induced Liver Injury physiopathology, China, Emulsions, Lipid Peroxidation, Liver drug effects, Liver metabolism, Liver pathology, Liver physiopathology, Lutein administration & dosage, Lutein chemistry, Lutein isolation & purification, Male, Malondialdehyde metabolism, Mice, Organ Size drug effects, Oxidoreductases antagonists & inhibitors, Oxidoreductases chemistry, Oxidoreductases metabolism, Random Allocation, Thylakoids chemistry, Algal Proteins therapeutic use, Antioxidants therapeutic use, Biological Products therapeutic use, Chemical and Drug Induced Liver Injury prevention & control, Chlorella vulgaris chemistry, Dietary Supplements, Lutein therapeutic use
- Abstract
A novel kind of lutein-protein complex (LPC) was extracted from heterotrophic Chlorella vulgaris through aqueous extraction. The purification procedure contained solubilization of thylakoid proteins by a zwitterionic detergent CHAPS, anion exchange chromatography and gel filtration chromatography. Both wavelength scanning and HPLC analysis confirmed that lutein was the major pigment of the protein-based complex, and the mass ratio of lutein and protein was determined to be 9.72 : 100. Besides showing lipid peroxidation inhibition activity in vitro, LPC exerted significant antioxidant effects against ABTS and DPPH radicals with IC50 of 2.90 and 97. 23 μg mL(-1), respectively. Meanwhile, in vivo antioxidant activity of the complex was evaluated using the mice hepatotoxicity model; LPC significantly suppressed the carbon tetrachloride-induced elevation of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, and decreased hepatic malondialdehyde (MDA) levels and the hepatosomatic index. Moreover, LPC could effectively restore the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in the treated mice livers. Our findings further the progress in the research of natural protein-based lutein complexes, suggesting that LPC has the potential in hepatoprotection against chemical induced toxicity and in increasing the antioxidant capacity of the defense system in the human body.
- Published
- 2015
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