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The alcohol precipitation wastewater discharged from the production of Chinese patent medicine (CPM) has an extremely high chemical oxygen demand (COD) and poor biodegradability. In this study, the biological treatment method of anaerobic digestion coupled with simultaneous nitrification and denitrification (SND) was adopted to investigate its efficiency and to explore the mechanism of pollutant degradation in this process. The results showed that after 220 days of debugging, the coupled process operated stably. The influent COD, total nitrogen (TN), ammonium (NH4+-N), and lignin concentrations were 21,000 mg/L, 400 mg/L, 200 mg/L, and 1800 mg/L, respectively. The removal efficiencies of COD, TN, NH4+-N, and lignin were 97%, 85%, 96%, and 75%, respectively. Spectral detection technology analysis revealed that the wastewater contained alkanes, olefins, phenols, alcohols, unsaturated organics, aromatic compounds, and humic acids. After the treatment by each unit of the process, the three-dimensional fluorescence intensity decreased by 86%; the standard volume of fluorescence area integration declined by 78%; the stretching vibration band of aromatic compounds showed peak splitting; and the molecular weight parameter value in the ultraviolet region increased. These findings demonstrated that the humic acid substances in the wastewater were degraded, and the effect of removal of the macromolecular organic matter was remarkable. [ABSTRACT FROM AUTHOR]

In this study, anaerobic digestion was employed to degrade the refractory, high-salinity PO/MTBE (propylene oxide/methyl tertiary butyl ether) manufacturing wastewater (P/MMW) in sequencing batch mode. H2SO4 was added into the system to neutralize the alkalinity produced by the hydrolysis of sodium formate in P/MMW. The pH value in reactor A was only adjusted at the beginning of each cycle, and controlled in the range of 7.51–8.26. Meanwhile, the pH value in reactor B was maintained at 7.2 by regulating multiple times throughout the acclimation cycles. After five cycles of acclimation, both reactors exhibited stable removal of chemical oxygen demand (COD), while COD removal in system B was significantly improved in later stages (cycle III–V). More sulfate was reduced to sulfide alongside the COD removal process in reactor B, indicating more organic pollutants were removed by sulfate-reducing bacteria. High-throughput sequencing results illustrated that Firmicutes and other microorganisms capable of degrading the main pollutants in P/MMW were enriched to a greater degree in reactor B. Microbial communities in reactors A and B were different from seed sludge and evolved into two distinct groups, with sulfate and sulfide concentrations being main contributing factors for microbial community selection. [ABSTRACT FROM AUTHOR]

According to the characteristics of aniline dyeing wastewater, aerobic biological treatment was adopted herein, and high-throughput sequencing technology was used to analyze the changes to the microbial community structure and diversity in the aerobic sludge acclimation stage. As a result, according to the experimental study on the effects of different biomass, the initial pH value and the salinity with the aerobic biological treatment, the chemical oxygen demand (COD) degradation rate can increase linearly with the increase in biomass under different biomass conditions. The organic matter degradation rate is 6.24 mg/L COD·h−1·(mg/L·MLSS)−1, with a correlation coefficient (R2) of 0.98704. When the initial pH value is less than 7.0 ± 0.2, the COD degradation rate increases with the increase in the initial pH value and then decreases gradually. The optimal sludge concentration is 4 g/L; the optimal initial pH value is in the range of 7.0–8.0; the optimal salinity is 1.7%. When the initial concentration of COD is 3000 mg/L, the COD value gradually stabilizes and decreases to 1500 mg/L after 32 h, the degradation rate reaches 50%, and the pH decreases from 7.5 to 4.5. Sphingopyxis has been detected in sludge samples from the third cycle of acclimation, which can biodegrade aromatic compounds, anthraquinone dyes, and their intermediates, and the relative abundance of Sphingopyxis increased from 0.18% to 5.08%, indicating a potential biodegradation ability of aniline dyeing wastewater. [ABSTRACT FROM AUTHOR]

Cometabolism technology was employed to degrade low-strength coking wastewater (CWW) in Sequencing Batch Reactor (SBR). The bacterial community compositions were monitored by high-throughput sequencing. Cometabolic substrate effectively improved the chemical oxygen demand (COD) removal efficiency in glucose-added system (A1) compared to glucose-free system (A0). Meanwhile, A1 exhibited larger biomass, better settlement performance, and higher dehydrogenase activity (DHA). More importantly, high-throughput sequencing revealed that dominant populations in A1 were quite different with A0. Thauera (9.27%), Thermogutta (7.58%), and Defluviimonas (4.6%) began to enrich in A1 after cometabolic substrate supplement. Especially, Thauera , as the most dominant populations in Al, could degrade a wide spectrum of aromatic compounds, which may contribute to the better system performance. This work would provide a novel option to treat low-strength CWW, discern the relationship between bacterial community and CWW quality, and further explore the cometabolic degradation through bacterial community structures. [ABSTRACT FROM AUTHOR]

• MCOR can effectively remove the organic pollutants from SSW. • Bacterial communities at salinities of 3.5% and 7.0% evolved into different groups. • Pseudomonas dominated at 3.5% salinity, while Marinobacterium enriched at 7.0% salinity. • Salinity drastically changed bacterial community. In this study, multistage contact oxidation reactor (MCOR) with a novel carrier was used for treatment of high-strength sodium saccharin wastewater (SSW) under stepwise increasing salinities from 1.0% to 8.0%. The results revealed that MCOR could effectively remove the organic pollutants from SSW when influent salinity was no more than 4.5%; the chemical oxygen demand (COD) and NH 4 +-N removal efficiency under the optimal operating parameters ranged up to 91.5% and 92.7%, respectively. Microbial diversity analysis illustrated that the dominant microbes in SSW treatment system were substantially distinct at different salinities. Pseudomonas was predominant at salinity of 3.5%, while Marinobacterium (a species involved in COD removal) was enriched to a greater degree at salinity of 7.0%. CCA suggested that salinity was the main factor for dynamic evolutions of microbial community structures. This work demonstrated that MCOR is an appropriate method for the treatment of high-strength, high-salinity SSW. [ABSTRACT FROM AUTHOR]