1. Determination of Polysaccharides in Undaria pinnatifida by Ionic Liquid-Modified Silica Gel Size Exclusion Chromatography.
- Author
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Lee, Yu Ri, Ma, Wanwan, and Row, Kyung Ho
- Subjects
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LIQUID chromatography , *POLYSACCHARIDES , *SILICA , *CHEMICAL synthesis , *AMINO group - Abstract
Ionic liquid-modified silica particles with a large pore size were synthesized and used as the stationary phase for size exclusion chromatography coupled with a refractive index detector for the determination of polysaccharides, such as fucoidan, alginic acid, and laminarin from Undaria pinnatifida (seaweed). The molecular weight of polysaccharide was determined by a dextran standard curve (5-1100 kDa). The ionic liquid-modified silica column exhibited excellent size exclusion properties in separating laminarin from other components. The 1-butyl-3-methyl-imidazolium bis-(trifluoromethylsulfonyl)-amino silica column has superior resolution in laminarin separation than the other columns because the amino-group in ionic liquid provide π-π interactions due to aromaticity of the ring structure, which enhances the effect of the hydroxyl group in the target compound separation. The concentrations of polysaccharides were calculated using a standard linear equation to be 0.332-0.484 mg/g of fucoidan, 0.207-0.301 mg/g of alginic acid, and 0.154-0.297 mg/g of laminarin. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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