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The effect of ultrasound frequency on the pulsed sonolytic degradation of octyl benzenesulfonate (OBS) is examined. The studies have shown that sonolytic degradation of OBS has depended on both the sonochemical activity and the accumulation of OBS on cavitation bubble surfaces.

A solution of perchlorotriphenylmethyl triester (PTM-TE, a stable organic free radical) dissolved in hexamethyldisiloxane (HMDS) is encapsulated by using high-power ultrasound into a polymerized shell of bovine serum albumin (BSA). It is found that the method leads to an overall loss of EPR signal intensity from the washed suspension of microspheres and the encapsulated PTM-TE/HMDS solution is found to be sensitive to the partial pressure of oxygen in the muscle and tumor tissue of mice.

An ultrasound-independent frequency effect was observed, which was made possible by carefully choosing the 'reference' and 'test' sonochemical reactions. It is concluded that sodium 1-pentaanesulfonic acid (SPSo) and sodium dodecyl sulfate (SDS) to accumulate at the gas/solution interface of cavitation bubbles.